Abstract
In chicken embryos of different ages and in young chickens after hatching, neural elements reacting with antibodies generated against synthetic ovine corticotropin-releasing factor (CRF) were studied by means of the peroxidase-anti-peroxidase (PAP) technique at the lightmicroscopic level. CRF-immunoreactivity was first observed in perikarya located in the periventricular part of the hypothalamus on the 14th day of the incubation period. CRF-containing neural elements were detected on the same day of incubation in the external zone of the median eminence, but not in all investigated animals. In extrahypothalamic sites, immunoreactive perikarya were demonstrable in the central gray of the mesencephalon on the 15th day of incubation. Furthermore, immunoreactive cells appeared in other brain regions such as nucleus accumbens and dorsomedial nucleus of the thalamus after hatching. The present observations provide information regarding the functional development of the hypothalamo-hypophyseal-adrenal axis in the chick embryo.
| Original language | English |
|---|---|
| Pages (from-to) | 681-685 |
| Number of pages | 5 |
| Journal | Cell and Tissue Research |
| Volume | 244 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 1 1986 |
| Externally published | Yes |
Fingerprint
Keywords
- Brain
- Chicken embryo
- Corticotropin-releasing factor (CRF)
- Neuropeptide immunocytochemistry
- Ontogeny
ASJC Scopus subject areas
- Anatomy
- Clinical Biochemistry
- Cell Biology
Cite this
Ontogenetic development of corticotropin-releasing factor (CRF)-containing neural elements in the brain of the chicken during incubation and after hatching. / Józsa, Rita; Vigh, Sándor; Mess, Béla; Schally, Andrew V.
In: Cell and Tissue Research, Vol. 244, No. 3, 01.06.1986, p. 681-685.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ontogenetic development of corticotropin-releasing factor (CRF)-containing neural elements in the brain of the chicken during incubation and after hatching
AU - Józsa, Rita
AU - Vigh, Sándor
AU - Mess, Béla
AU - Schally, Andrew V
PY - 1986/6/1
Y1 - 1986/6/1
N2 - In chicken embryos of different ages and in young chickens after hatching, neural elements reacting with antibodies generated against synthetic ovine corticotropin-releasing factor (CRF) were studied by means of the peroxidase-anti-peroxidase (PAP) technique at the lightmicroscopic level. CRF-immunoreactivity was first observed in perikarya located in the periventricular part of the hypothalamus on the 14th day of the incubation period. CRF-containing neural elements were detected on the same day of incubation in the external zone of the median eminence, but not in all investigated animals. In extrahypothalamic sites, immunoreactive perikarya were demonstrable in the central gray of the mesencephalon on the 15th day of incubation. Furthermore, immunoreactive cells appeared in other brain regions such as nucleus accumbens and dorsomedial nucleus of the thalamus after hatching. The present observations provide information regarding the functional development of the hypothalamo-hypophyseal-adrenal axis in the chick embryo.
AB - In chicken embryos of different ages and in young chickens after hatching, neural elements reacting with antibodies generated against synthetic ovine corticotropin-releasing factor (CRF) were studied by means of the peroxidase-anti-peroxidase (PAP) technique at the lightmicroscopic level. CRF-immunoreactivity was first observed in perikarya located in the periventricular part of the hypothalamus on the 14th day of the incubation period. CRF-containing neural elements were detected on the same day of incubation in the external zone of the median eminence, but not in all investigated animals. In extrahypothalamic sites, immunoreactive perikarya were demonstrable in the central gray of the mesencephalon on the 15th day of incubation. Furthermore, immunoreactive cells appeared in other brain regions such as nucleus accumbens and dorsomedial nucleus of the thalamus after hatching. The present observations provide information regarding the functional development of the hypothalamo-hypophyseal-adrenal axis in the chick embryo.
KW - Brain
KW - Chicken embryo
KW - Corticotropin-releasing factor (CRF)
KW - Neuropeptide immunocytochemistry
KW - Ontogeny
UR - http://www.scopus.com/inward/record.url?scp=0022891149&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022891149&partnerID=8YFLogxK
U2 - 10.1007/BF00212549
DO - 10.1007/BF00212549
M3 - Article
C2 - 3487385
AN - SCOPUS:0022891149
VL - 244
SP - 681
EP - 685
JO - Cell and Tissue Research
JF - Cell and Tissue Research
SN - 0302-766X
IS - 3
ER -