One and four layer acellular bladder matrix for fascial tissue reconstruction

Daniel Eberli, Anthony Atala, James J. Yoo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To determine whether the use of multiple layers of acellular bladder matrix (ABM) is more suitable for the treatment of abdominal wall hernia than a single layered ABM. The feasibility, biocompatibility and mechanical properties of both materials were assessed and compared. Biocompatibility testing was performed on 4 and 1 layered ABM. The matrices were used to repair an abdominal hernia model in 24 rabbits. The animals were followed for up to 3 months. Immediately after euthanasia, the implant site was inspected and samples were retrieved for histology, scanning electron microscopy and biomechanical studies. Both acellular biomaterials demonstrated excellent biocompatibility. At the time of retrieval, there was no evidence of infection. The matrices demonstrated biomechanical properties comparable to native tissue. Three hernias (25%) were found in the single layer ABM group and only 1 hernia (8%) was found in the 4 layer ABM group. Histologically, the matrix structure was intact and the cell density within the matrices decreased with time. The dominant cell type present within the matrices shifted from lymphocytes to fibroblasts over time. Both ABMs maintained adequate strength over time when used for hernia repair, and there was an extremely low incidence of adhesion formation. The single layer ABM showed enhanced cellular integration, while the 4 layer ABM reduced hernia formation. Either of these matrices may be useful as an off-the-shelf biomaterial for patients requiring fascial repair.

Original languageEnglish
Pages (from-to)741-751
Number of pages11
JournalJournal of Materials Science: Materials in Medicine
Volume22
Issue number3
DOIs
StatePublished - Mar 1 2011
Externally publishedYes

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Urinary Bladder
Tissue
Hernia
Abdominal Hernia
Biocompatible Materials
Biocompatibility
Materials Testing
Repair
Euthanasia
Herniorrhaphy
Abdominal Wall
Biomaterials
Electron Scanning Microscopy
Histology
Fibroblasts
Cell Count
Lymphocytes
Rabbits
Incidence
Infection

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Cite this

One and four layer acellular bladder matrix for fascial tissue reconstruction. / Eberli, Daniel; Atala, Anthony; Yoo, James J.

In: Journal of Materials Science: Materials in Medicine, Vol. 22, No. 3, 01.03.2011, p. 741-751.

Research output: Contribution to journalArticle

Eberli, Daniel ; Atala, Anthony ; Yoo, James J. / One and four layer acellular bladder matrix for fascial tissue reconstruction. In: Journal of Materials Science: Materials in Medicine. 2011 ; Vol. 22, No. 3. pp. 741-751.
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