On the mechanism of erythroid cell sensitivity to chloramphenicol: Studies on mitochondria isolated from erythroid and myeloid tumors

Samir Abou-Khalil, Ziad Salem, Wafa H. Abou-Khalil, Adel A. Yunis

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Abstract

Erythroleukemia mitochondria (E. Mito) and chloroma mitochondria (C. Mito) were isolated from tumors grown in their hosts, DBA/2J mice and Long-Evans rats, respectively. Oxypolarographic tests showed respiratory control and ADP/O ratios typical for well-coupled mitochondria. Therapeutic concentration of chloramphenicol (CAP) had no effect on the energy transfer of those mitochondria. l-[14C]leucine incorporation into protein was comparable in both types of mitochondria. Although the incorporation at 15 min appeared higher in C. Mito, at 60 min it became similar to that in E. Mito. When CAP was used at the therapeutic concentration of 20 μg/ml about 80% inhibition was observed in both mitochondria. The exogenous amino acid mixture added to the medium was an important determinant in both the rate of leucine incorporation as well as the sensitivity to CAP. Thus, if no amino acids were added the incorporation was reduced to 18-25%. Under these conditions, however E. Mito were significantly more sensitive to the same concentration of CAP than C. Mito. The results suggest that mitochondrial amino acid pool may be involved in the greater sensitivity of erythroid precursors to CAP.

Original languageEnglish (US)
Pages (from-to)242-248
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume206
Issue number2
DOIs
StatePublished - Feb 1981

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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