Oligoribonuclease is encoded by a highly conserved gene in the 3'-5' exonuclease superfamily

Xiaolan Zhang, Liuqin Zhu, Murray P Deutscher

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Oligoribonuclease, a 3'-to-5' exoribonuclease specific for small oligoribonucleotides, was purified to homogeneity from extracts of Escherichia coli. The purified protein is an α2 dimer of 40 kDa. NH2- terminal sequence analysis of the protein identified the gene encoding oligoribonuclease as yjeR (o204a), a previously reported open reading frame located at 94 min on the E. coli chromosome. However, as a consequence of the sequence information, the translation start site of this open reading frame has been revised. Cloning of yjeR led to overexpression of oligoribonuclease activity, and interruption of the cloned gene with a kanamycin resistance cassette eliminated the overexpression. On the basis of these data, we propose that yjeR be renamed orn. Orthologs of oligoribonuclease are present in a wide range of organisms, extending up to humans.

Original languageEnglish
Pages (from-to)2779-2781
Number of pages3
JournalJournal of Bacteriology
Volume180
Issue number10
StatePublished - May 1 1998

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Exonucleases
Open Reading Frames
Genes
Oligoribonucleotides
Kanamycin Resistance
Escherichia coli
Protein Sequence Analysis
Organism Cloning
Chromosomes
oligoribonuclease
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Oligoribonuclease is encoded by a highly conserved gene in the 3'-5' exonuclease superfamily. / Zhang, Xiaolan; Zhu, Liuqin; Deutscher, Murray P.

In: Journal of Bacteriology, Vol. 180, No. 10, 01.05.1998, p. 2779-2781.

Research output: Contribution to journalArticle

Zhang, X, Zhu, L & Deutscher, MP 1998, 'Oligoribonuclease is encoded by a highly conserved gene in the 3'-5' exonuclease superfamily', Journal of Bacteriology, vol. 180, no. 10, pp. 2779-2781.
Zhang, Xiaolan ; Zhu, Liuqin ; Deutscher, Murray P. / Oligoribonuclease is encoded by a highly conserved gene in the 3'-5' exonuclease superfamily. In: Journal of Bacteriology. 1998 ; Vol. 180, No. 10. pp. 2779-2781.
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