Oligoribonuclease is distinct from the other known exoribonucleases of Escherichia coli

D. Yu, Murray P Deutscher

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enzymes, that oligoribonuclease is not a manifestation of RNases D, BN, T, PH, and R, exoribonucleases discovered subsequently. Thus, oligoribonuclease is the eighth distinct exoribonuclease discovered in Escherichia coli. We also show that oligoribonuclease copurifies with polynucleotide phosphorylase.

Original languageEnglish
Pages (from-to)4137-4139
Number of pages3
JournalJournal of Bacteriology
Volume177
Issue number14
StatePublished - Jul 26 1995

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Exoribonucleases
Polyribonucleotide Nucleotidyltransferase
Escherichia coli
Ribonuclease III
Oligoribonucleotides
oligoribonuclease
Enzymes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Oligoribonuclease is distinct from the other known exoribonucleases of Escherichia coli. / Yu, D.; Deutscher, Murray P.

In: Journal of Bacteriology, Vol. 177, No. 14, 26.07.1995, p. 4137-4139.

Research output: Contribution to journalArticle

Yu, D. ; Deutscher, Murray P. / Oligoribonuclease is distinct from the other known exoribonucleases of Escherichia coli. In: Journal of Bacteriology. 1995 ; Vol. 177, No. 14. pp. 4137-4139.
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