Oligoribonuclease is an essential component of the mRNA decay pathway

Sandip Ghosh, Murray P Deutscher

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

mRNA decay in prokaryotic cells involves the action of both endo- and exoribonucleases. In Escherichia coli, degradation of RNA to the mononucleotide level was thought to depend on RNase II and polynucleotide phosphorylase. Here, we show that the enzyme oligoribonuclease is an essential part of this process as well. Thus, inactivation of the orn gene encoding oligoribonuclease leads to a cessation of cell growth. Moreover, although pulse-labeled RNA decays normally in orn mutant cells under nonpermissive conditions, a large fraction of the resulting products is small oligoribonucleotides rather than the mononucleotides generated in wildtype cells. The oligoribonucleotides that accumulate are 2-5 residues in length; longer molecules disappear during the decay process. These data indicate that oligoribonuclease is required to complete the degradation of mRNA to mononucleotides and that this process is required for cell viability. Inasmuch as close homologues of the orn gene are found in a wide range of eukaryotes, extending up to humans, these findings raise the possibility that oligoribonuclease also participates in mRNA degradation in these organisms.

Original languageEnglish
Pages (from-to)4372-4377
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number8
DOIs
StatePublished - Apr 13 1999

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RNA Stability
Oligoribonucleotides
Exoribonucleases
Polyribonucleotide Nucleotidyltransferase
Prokaryotic Cells
Endonucleases
Gene Silencing
Eukaryota
Cell Survival
oligoribonuclease
Escherichia coli
Enzymes
Growth
Genes

ASJC Scopus subject areas

  • Genetics
  • General

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Oligoribonuclease is an essential component of the mRNA decay pathway. / Ghosh, Sandip; Deutscher, Murray P.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 8, 13.04.1999, p. 4372-4377.

Research output: Contribution to journalArticle

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