Oligoclonal bands in unconcentrated CSF - Silver stain for agarose gels

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Agarose gel electrophoresis (AGE) and agarose isoelectric focusing (AIEF) were performed on unconcentrated CSF from patients with multiple sclerosis and other neurological disorders. Electrophoretic profiles were examined after staining with silver. Oligoclonal distribution of immunoglobulins were observed in over 90% of patients with clinically definite multiple sclerosis and over half of all patients with possible or probable multiple sclerosis. Up to 20 samples could be simultaneously examined and required the use of only 2-20 μL of unconcentrated CSF containing 10-100 ng of total IgG. The entire procedure from sample application to completion of staining required less than 3 h. The techniques offered exquisite resolution of abnormal immunoglobulin bands and permitted distinction of normal from abnormal CSF readily.

Original languageEnglish
Pages (from-to)15-25
Number of pages11
JournalNeurochemical Pathology
Volume3
Issue number1
DOIs
StatePublished - Mar 1 1983
Externally publishedYes

Fingerprint

Oligoclonal Bands
Silver
Sepharose
Multiple Sclerosis
Coloring Agents
Gels
Silver Staining
Agar Gel Electrophoresis
Isoelectric Focusing
Nervous System Diseases
Immunoglobulins
Immunoglobulin G
Staining and Labeling

Keywords

  • CSF immunoglobulins
  • CSF immunoglobulins, electrophoresis of
  • immunoglobulins, in CSF
  • isoelectric focusing, of CSF immunoglobulins
  • multiple sclerosis diagnosis, by electrophoresis of
  • oligoclonal bands, in CSF immunoglobulin

ASJC Scopus subject areas

  • Clinical Neurology
  • Molecular Biology

Cite this

Oligoclonal bands in unconcentrated CSF - Silver stain for agarose gels. / Rammohan, Kottil W.

In: Neurochemical Pathology, Vol. 3, No. 1, 01.03.1983, p. 15-25.

Research output: Contribution to journalArticle

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