OLIG2 (BHLHB1), a bHLH transcription factor, contributes to leukemogenesis in concert with LMO1

Ying Wei Lin, Ramona Deveney, Mary Barbara, Norman N. Iscove, Stephen D Nimer, Christopher Slape, Peter D. Aplan

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

OLIG2 (originally designated BHLHB1) encodes a transcription factor that contains the basic helix-loop-helix motif. Although expression of OLIG2 is normally restricted to neural tissues, overexpression of OLIG2 has been shown in patients with precursor T-cell lymphoblastic lymphoma/leukemia (pre-T LBL). In the current study, we found that overexpression of OLIG2 was not only found in oligodendroglioma samples and normal neural tissue but also in a wide spectrum of malignant cell lines including leukemia, non-small cell lung carcinoma, melanoma, and breast cancer cell lines. To investigate whether enforced expression of OLIG2 is oncogenic, we generated transgenic mice that overexpressed OLIG2 in the thymus. Ectopic OLIG2 expression in the thymus was only weakly oncogenic as only 2 of 85 mice developed pre-T LBL. However, almost 60% of transgenic mice that overexpressed both OLIG2 and LMO1 developed pre-T LBL with large thymic tumor masses. Gene expression profiling of thymic tumors that developed in OLIG2/LMO1 mice revealed up-regulation of Notch1 as well as Deltex1 (Dtx1) and pre T-cell antigen receptor α (Ptcrα), two genes that are considered to be downstream of Notch1. Of note, we found mutations in the Notch1 heterodimerization or proline-, glutamic acid-, serine-, and threonine-rich domain in three of six primary thymic tumors. In addition, growth of leukemic cell lines established from OLIG2/LMO1 transgenic mice was suppressed by a γ-secretase inhibitor, suggesting that Notch1 up-regulation is important for the proliferation of OLIG2-LMO1 leukemic cells.

Original languageEnglish
Pages (from-to)7151-7158
Number of pages8
JournalCancer Research
Volume65
Issue number16
DOIs
StatePublished - Aug 15 2005
Externally publishedYes

Fingerprint

Thymus Neoplasms
Basic Helix-Loop-Helix Transcription Factors
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Transgenic Mice
Cell Line
Thymus Gland
Helix-Loop-Helix Motifs
Up-Regulation
Oligodendroglioma
Amyloid Precursor Protein Secretases
Gene Expression Profiling
Threonine
T-Cell Antigen Receptor
Proline
Non-Small Cell Lung Carcinoma
Serine
Glutamic Acid
Melanoma
Leukemia
Transcription Factors

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

OLIG2 (BHLHB1), a bHLH transcription factor, contributes to leukemogenesis in concert with LMO1. / Lin, Ying Wei; Deveney, Ramona; Barbara, Mary; Iscove, Norman N.; Nimer, Stephen D; Slape, Christopher; Aplan, Peter D.

In: Cancer Research, Vol. 65, No. 16, 15.08.2005, p. 7151-7158.

Research output: Contribution to journalArticle

Lin, Ying Wei ; Deveney, Ramona ; Barbara, Mary ; Iscove, Norman N. ; Nimer, Stephen D ; Slape, Christopher ; Aplan, Peter D. / OLIG2 (BHLHB1), a bHLH transcription factor, contributes to leukemogenesis in concert with LMO1. In: Cancer Research. 2005 ; Vol. 65, No. 16. pp. 7151-7158.
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AU - Slape, Christopher

AU - Aplan, Peter D.

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