Abstract
The bone marrow of old adult mice (∼2 years old) has reduced B lymphopoiesis; however, whether the B1 pathway in adult bone marrow is also compromised in senescence is not known. Herein, we show that phenotypic (IgM-Lin-CD93+[AA4.1+] CD19+B220low/-) B1 progenitors are retained in old bone marrow even as B2 B cell precursors are reduced. Moreover, B1 progenitors from both young adult and old mice generated new B cells in vitro enriched for CD43 expression, likely due to their activation, and exhibited increased λ light chain usage and diminished levels of κ light chain expression. B1 progenitors were shown to have lower surrogate light chain (λ5) protein levels than did B2 pro-B cells in young mice and these levels decreased in both B1 and B2 precursor pools in old age. These results indicate that the B1 B cell pathway persists during old age in contrast to the B2 pathway. Moreover, B1 B cell progenitors generated new B cells in the adult bone marrow that have distinct surface phenotype and light chain usage. This is associated with decreased surrogate light chain expression, a characteristic held in common by B1 progenitors as well as B2 precursors in old mice.
Original language | English (US) |
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Pages (from-to) | 401-408 |
Number of pages | 8 |
Journal | Mechanisms of Ageing and Development |
Volume | 130 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2009 |
Keywords
- B cell precursors
- B1 B cells
- Light chain
- Lymphopoiesis
- Senescence
- Surrogate light chain
ASJC Scopus subject areas
- Aging
- Developmental Biology