Odorant receptor from the Southern House mosquito narrowly tuned to the oviposition attractant skatole

David T. Hughes, Julien Pelletier, Charles W. Luetje, Walter S. Leal

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Oviposition attractants are environmental cues that allow Culex gravid female mosquitoes to locate suitable sites for egg-laying and, therefore, may be exploited for environmentally friendly strategies for controlling mosquito populations. Naturally occurring skatole has been identified as an oviposition attractant for the Southern House mosquito, Culex quinquefasciatus. Previously, we identified in Cx. quinquefasciatus female antennae an olfactory receptor neuron (ORN) highly sensitive to skatole and an odorant-binding protein involved in the detection of this semiochemical. Here, we describe the characterization of an odorant receptor (OR), CquiOR10, which is narrowly tuned to skatole when expressed in the Xenopus oocyte system. Odorant-induced response profiles generated by heterologously expressed CquiOR10 suggest that this OR is expressed in the mosquito ORN sensitive to skatole. However, geranylacetone, which stimulates the antennal ORN, was not detected by CquiOR10-expressing oocytes, thus raising interesting questions about reception of oviposition attractants in mosquitoes.

Original languageEnglish (US)
Pages (from-to)797-800
Number of pages4
JournalJournal of Chemical Ecology
Volume36
Issue number8
DOIs
StatePublished - 2010

Keywords

  • 2-Methylphenol
  • 3-Methylindole
  • CquiOR10
  • Culex quinquefasciatus
  • Odorant receptor
  • Xenopus oocyte expression system

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

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