Octreotide Attenuates Acute Kidney Injury after Hepatic Ischemia and Reperfusion by Enhancing Autophagy

Huiping Sun, Shuangfa Zou, Keith A Candiotti, Yanhua Peng, Qinya Zhang, Weiqiang Xiao, Yiyun Wen, Jiao Wu, Jinfeng Yang

Research output: Contribution to journalArticle

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Abstract

Octreotide exerts a protective effect in hepatic ischemia-reperfusion (HIR) injury. However, whether octreotide preconditioning could also reduce acute kidney injury (AKI) after HIR is unknown. This study was designed to investigate the role of octreotide in AKI after HIR. Male Sprague-Dawley rats were pretreated with octreotide or octreotide combined with 3-methyladenine (autophagy inhibitor, 3MA). Plasma creatinine, inflammation markers (e.g., TNF-α and IL-6 etc.), apoptosis, autophagy and phosphorylation of protein kinase B/mammalian target of rapamycin/p70 ribosomal S6 kinase (Akt/mTOR/p70S6K) in the kidney were measured after 60 minutes of liver ischemia and 24 hours of reperfusion for each rat. Octreotide pretreatment significantly preserved renal function and reduced the severity of renal injury. Moreover, octreotide alleviated inflammation and apoptosis in the kidney after HIR. Additionally, octreotide induced autophagy and autophagy inhibition with 3MA markedly reversed the renoprotective, anti-inflammatory and anti-apoptotic effects of octreotide after HIR. Finally, octreotide abrogated the activation of phosphorylation of Akt, mTOR and p70S6K in the kidney after HIR. Our results indicate that octreotide reduced renal injury after HIR due to its induction of autophagy. The enhancement of autophagy may be potentially linked to the octreotide mediated Akt/mTOR/p70S6K pathway deactivation and reduction of kidney inflammation and apoptosis after HIR.

Original languageEnglish (US)
Article number42701
JournalScientific Reports
Volume7
DOIs
StatePublished - Feb 16 2017

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Octreotide
Autophagy
Acute Kidney Injury
Reperfusion
Ischemia
Liver
70-kDa Ribosomal Protein S6 Kinases
Kidney
Apoptosis
Inflammation
Phosphorylation
Proto-Oncogene Proteins c-akt
Wounds and Injuries
Sirolimus
Reperfusion Injury
Sprague Dawley Rats
Interleukin-6
Creatinine
Anti-Inflammatory Agents

ASJC Scopus subject areas

  • General

Cite this

Octreotide Attenuates Acute Kidney Injury after Hepatic Ischemia and Reperfusion by Enhancing Autophagy. / Sun, Huiping; Zou, Shuangfa; Candiotti, Keith A; Peng, Yanhua; Zhang, Qinya; Xiao, Weiqiang; Wen, Yiyun; Wu, Jiao; Yang, Jinfeng.

In: Scientific Reports, Vol. 7, 42701, 16.02.2017.

Research output: Contribution to journalArticle

Sun, Huiping ; Zou, Shuangfa ; Candiotti, Keith A ; Peng, Yanhua ; Zhang, Qinya ; Xiao, Weiqiang ; Wen, Yiyun ; Wu, Jiao ; Yang, Jinfeng. / Octreotide Attenuates Acute Kidney Injury after Hepatic Ischemia and Reperfusion by Enhancing Autophagy. In: Scientific Reports. 2017 ; Vol. 7.
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