Obtaining patient-derived cancer organoid cultures via fine-needle aspiration

Courtney J. Phifer, Kensey N. Bergdorf, Matthew E. Bechard, Anna Vilgelm, Naira Baregamian, Oliver G. McDonald, Ethan Lee, Vivian L. Weiss

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Patient-derived tumor organoid cultures are an essential and innovative methodology for translational research. However, current techniques to establish these cultures are cumbersome, expensive, and often require irreplaceable clinical tissue from surgery or core biopsies. Fine-needle aspiration (FNA) provides a minimally invasive biopsy technique commonly performed in clinical settings. Here, we provide a protocol for FNA. We have found that FNA provides a cost-effective, rapid, and streamlined method for tissue acquisition for cancer organoid culture. For complete details on the use and execution of this protocol, please refer to Lee et al. (2020) and Vilgelm et al. (2020).

Original languageEnglish (US)
Article number100220
JournalSTAR Protocols
Issue number1
StatePublished - Mar 19 2021
Externally publishedYes


  • cancer
  • cell culture
  • cell isolation
  • organoids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Medicine(all)


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