Obtaining Human Breast Adipose Cells for Breast Cancer Cell Co-culture Studies

Manuel Picon-Ruiz, Juan A. Marchal, Joyce M. Slingerland

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Primary human breast cancers invade surrounding fat and contact adipocytes, inflammatory infiltrates, and fibrous stroma. This tissue niche influences breast tumor progression. Here, we present a protocol to enable the in vitro study of the complex interactions that occur between breast cancer cells and adipose cells. We describe how to obtain different adipose cell populations, including adipose-derived stem cells, immature adipocytes, and mature adipocytes, from human breast fat tissue and detail the application for co-culture assays with breast cancer cells. For complete details on the use and execution of this protocol, please refer to Picon-Ruiz et al. (2016) and Qureshi et al. (2020).

Original languageEnglish (US)
Article number100197
JournalSTAR Protocols
Issue number3
StatePublished - Dec 18 2020
Externally publishedYes


  • Cancer
  • Cell culture
  • Cell Differentiation
  • Cell isolation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)


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