Objective comparison of particle tracking methods

Nicolas Chenouard, Ihor Smal, Fabrice De Chaumont, Martin Maška, Ivo F. Sbalzarini, Yuanhao Gong, Janick Cardinale, Craig Carthel, Stefano Coraluppi, Mark Winter, Andrew R. Cohen, William J. Godinez, Karl Rohr, Yannis Kalaidzidis, Liang Liang, James Duncan, Hongying Shen, Yingke Xu, Klas E.G. Magnusson, Joakim JaldénHelen M. Blau, Perrine Paul-Gilloteaux, Philippe Roudot, Charles Kervrann, François Waharte, Jean Yves Tinevez, Spencer L. Shorte, Joost Willemse, Katherine Celler, Gilles P. Van Wezel, Han Wei Dan, Yuh Show Tsai, Carlos Ortiz De Solórzano, Jean Christophe Olivo-Marin, Erik Meijering

Research output: Contribution to journalArticlepeer-review

515 Scopus citations


Particle tracking is of key importance for quantitative analysis of intracellular dynamic processes from time-lapse microscopy image data. Because manually detecting and following large numbers of individual particles is not feasible, automated computational methods have been developed for these tasks by many groups. Aiming to perform an objective comparison of methods, we gathered the community and organized an open competition in which participating teams applied their own methods independently to a commonly defined data set including diverse scenarios. Performance was assessed using commonly defined measures. Although no single method performed best across all scenarios, the results revealed clear differences between the various approaches, leading to notable practical conclusions for users and developers.

Original languageEnglish (US)
Pages (from-to)281-289
Number of pages9
JournalNature Methods
Issue number3
StatePublished - Mar 2014
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Objective comparison of particle tracking methods'. Together they form a unique fingerprint.

Cite this