Numerical simulations of cell flow and trapping within microfluidic channels for stiffness based cell isolation

Mutabe S. Aljaghtham, Zixiang L. Liu, Jing J. Guo, Jin He, Emrah Celik

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Analysis of rare cells in heterogenous mixtures is proven to be beneficial for regenerative medicine, cancer treatment and prenatal diagnostics. Scarcity of these cells, however, makes the isolation process extremely challenging. Efficiency in cell isolation is still low and therefore, novel cell isolation strategies with new biomarkers need exploration. In this study, we investigated the feasibility of using the mechanical stiffness difference to detect and isolate the rare cells from the surrounding cells without labelling them. Fluid and solid mechanics simulations have shown that cell isolation can be performed at high efficiency using stiffness-based isolation. Accuracy of the numerical simulations is established using microfluidic flow chamber experiments.

Original languageEnglish (US)
JournalJournal of Biomechanics
DOIs
StateAccepted/In press - Jan 1 2019

Keywords

  • Cancer
  • Cell isolation
  • Deformation
  • Finite element analysis
  • Stiffness

ASJC Scopus subject areas

  • Biophysics
  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Rehabilitation

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