Abstract
Multinucleated skeletal muscle fibers synthesize cell surface and secreted oligomeric forms of acetylcholinesterase (AChE) that accumulate at specialized locations on the cell surface, such as sites of nerve-muscle contact. Using allelic variants of the AChE polypeptide chains as genetic markers, we show that nuclei homozygous for either the α or β alleles residing in chimeric myotubes preferentially translate their AChE mRNAs on their respective ERs. These results indicate that the events of transcription, translation, and assembly of this membrane protein are compartmentalized into nuclear domains in multinucleated cells, and provide the structural basis for the possible localized expression and regulation of synaptic components at the neuromuscular junctions of vertebrate skeletal muscle fibers.
| Original language | English |
|---|---|
| Pages (from-to) | 715-719 |
| Number of pages | 5 |
| Journal | Journal of Cell Biology |
| Volume | 110 |
| Issue number | 3 |
| State | Published - Mar 1 1990 |
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ASJC Scopus subject areas
- Cell Biology
Cite this
Nucleus-specific translation and assembly of acetylcholinesterase in multinucleated muscle cells. / Rotundo, Richard L; Gomez, Anna M.
In: Journal of Cell Biology, Vol. 110, No. 3, 01.03.1990, p. 715-719.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nucleus-specific translation and assembly of acetylcholinesterase in multinucleated muscle cells
AU - Rotundo, Richard L
AU - Gomez, Anna M.
PY - 1990/3/1
Y1 - 1990/3/1
N2 - Multinucleated skeletal muscle fibers synthesize cell surface and secreted oligomeric forms of acetylcholinesterase (AChE) that accumulate at specialized locations on the cell surface, such as sites of nerve-muscle contact. Using allelic variants of the AChE polypeptide chains as genetic markers, we show that nuclei homozygous for either the α or β alleles residing in chimeric myotubes preferentially translate their AChE mRNAs on their respective ERs. These results indicate that the events of transcription, translation, and assembly of this membrane protein are compartmentalized into nuclear domains in multinucleated cells, and provide the structural basis for the possible localized expression and regulation of synaptic components at the neuromuscular junctions of vertebrate skeletal muscle fibers.
AB - Multinucleated skeletal muscle fibers synthesize cell surface and secreted oligomeric forms of acetylcholinesterase (AChE) that accumulate at specialized locations on the cell surface, such as sites of nerve-muscle contact. Using allelic variants of the AChE polypeptide chains as genetic markers, we show that nuclei homozygous for either the α or β alleles residing in chimeric myotubes preferentially translate their AChE mRNAs on their respective ERs. These results indicate that the events of transcription, translation, and assembly of this membrane protein are compartmentalized into nuclear domains in multinucleated cells, and provide the structural basis for the possible localized expression and regulation of synaptic components at the neuromuscular junctions of vertebrate skeletal muscle fibers.
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M3 - Article
C2 - 2307705
AN - SCOPUS:0025371697
VL - 110
SP - 715
EP - 719
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 3
ER -