Nucleus-specific translation and assembly of acetylcholinesterase in multinucleated muscle cells

Richard L Rotundo, Anna M. Gomez

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Multinucleated skeletal muscle fibers synthesize cell surface and secreted oligomeric forms of acetylcholinesterase (AChE) that accumulate at specialized locations on the cell surface, such as sites of nerve-muscle contact. Using allelic variants of the AChE polypeptide chains as genetic markers, we show that nuclei homozygous for either the α or β alleles residing in chimeric myotubes preferentially translate their AChE mRNAs on their respective ERs. These results indicate that the events of transcription, translation, and assembly of this membrane protein are compartmentalized into nuclear domains in multinucleated cells, and provide the structural basis for the possible localized expression and regulation of synaptic components at the neuromuscular junctions of vertebrate skeletal muscle fibers.

Original languageEnglish
Pages (from-to)715-719
Number of pages5
JournalJournal of Cell Biology
Volume110
Issue number3
StatePublished - Mar 1 1990

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Skeletal Muscle Fibers
Acetylcholinesterase
Muscle Cells
Neuromuscular Junction
Genetic Markers
Vertebrates
Membrane Proteins
Alleles
Muscles
Messenger RNA
Peptides

ASJC Scopus subject areas

  • Cell Biology

Cite this

Nucleus-specific translation and assembly of acetylcholinesterase in multinucleated muscle cells. / Rotundo, Richard L; Gomez, Anna M.

In: Journal of Cell Biology, Vol. 110, No. 3, 01.03.1990, p. 715-719.

Research output: Contribution to journalArticle

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