Nucleotide metabolism in the whole blood of various vertebrates: enzyme levels and the use of high pressure liquid chromatography for the determination of nucleotide patterns

Phyllis R. Brown, Ram P. Agarwal, Jonathan Gell, R. E. Parks

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

1. 1. The nucleotide profiles of whole blood of man and animals were obtained by high pressure liquid chromatography. 2. 2. Within a species, the chromatograms were remarkably reproducible; however, each species exhibited a variation in the pattern that was characteristically its own. 3. 3. The major difference in higher and lower species was in the distinguishable 4. amounts of GTP and UTP in the lower species and the virtual absence of these compounds in the higher species. 5. 4. Adenylate kinase, guanlate kinase, nucleoside diphosphokinase and purine nucleoside phosphorylase activity were determined in the erythrocytes of some of these species.

Original languageEnglish
Pages (from-to)891-904
Number of pages14
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume43
Issue number4
DOIs
StatePublished - Dec 15 1972
Externally publishedYes

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Nucleoside-Diphosphate Kinase
High pressure liquid chromatography
Purine-Nucleoside Phosphorylase
Adenylate Kinase
Uridine Triphosphate
Guanosine Triphosphate
Metabolism
Vertebrates
Animals
Blood
Phosphotransferases
Nucleotides
Erythrocytes
High Pressure Liquid Chromatography
Enzymes

Keywords

  • adenalyte kinase
  • blood
  • chromattography
  • enzyme levels
  • guanylate kinase
  • high pressure liquid chromatography
  • liquid chromatography
  • nucleoside diphosphokinase
  • nucleotide metabolism purine metabolism
  • nucleotide patterns
  • nucleotide profiles
  • Nucleotides
  • purine nuceloside phosphorylase
  • vertebrate whole blood

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Nucleotide metabolism in the whole blood of various vertebrates : enzyme levels and the use of high pressure liquid chromatography for the determination of nucleotide patterns. / Brown, Phyllis R.; Agarwal, Ram P.; Gell, Jonathan; Parks, R. E.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 43, No. 4, 15.12.1972, p. 891-904.

Research output: Contribution to journalArticle

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