Nucleotide-mediated calcium signaling in rat cortical astrocytes: Role of P2X and P2Y receptors

Marta Fumagalli, Roberta Brambilla, Nadia D'Ambrosi, Cinzia Volonté, Michela Matteoli, Claudia Verderio, Maria P. Abbracchio

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

ATP is the dominant messenger for astrocyte-to-astrocyte calcium-mediated communication. Definition of the exact ATP/P2 receptors in astrocytes and of their coupling to intracellular calcium ([Ca2+]i) has important implications for brain physiology and pathology. We show that, with the only exception of the P2X6 receptor, primary rat cortical astrocytes express all cloned ligand-gated P2X (i.e., P2X1-5 and P2X7) and G-protein-coupled P2Y receptors (i.e., P2Y1, P2Y2, P2Y4, P2Y6, and P2Y12). These cells also express the P2Y-like UDP-glucose receptor, which has been recently recognized as the P2Y14 receptor. Single-cell image analysis showed that only some of these receptors are coupled to [Ca2+]i. While ATP induced rapid and transient [Ca2+]i increases (counteracted by the P2 antagonists suramin, pyridoxal-phosphate-6-azophenyl-2′-4′-disulfonic acid and oxidized ATP), the P2X1/P2X3 agonist αβmeATP produced no changes. Conversely, the P2X7 agonist BzATP markedly increased [Ca2+]i; the presence and function of the P2X7 receptor was also confirmed by the formation of the P2X7 pore. ADP and 2meSADP also produced [Ca2+]i increases antagonized by the P2Y1 antagonist MRS2179. Some cells also responded to UTP but not to UDP. Significant responses to sugar-nucleotides were also detected, which represents the first functional response reported for the putative P2Y14 receptor in a native system. Based on agonist preference of known P2 receptors, we conclude that, in rat astrocytes, ATP-induced calcium rises are at least mediated by P2X7 and P2Y1 receptors; additional receptors (i.e., P2X2, P2X4, P2X5, P2Y4, and P2Y14) may also contribute.

Original languageEnglish
Pages (from-to)218-230
Number of pages13
JournalGLIA
Volume43
Issue number3
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

Fingerprint

Calcium Signaling
Astrocytes
Nucleotides
Purinergic P2X7 Receptors
Adenosine Triphosphate
Calcium
Purinergic P2X2 Receptors
Purinergic P2Y1 Receptors
Single-Cell Analysis
Purinergic P2 Receptors
Uridine Diphosphate Glucose
Suramin
Uridine Triphosphate
Pyridoxal Phosphate
Uridine Diphosphate
G-Protein-Coupled Receptors
Adenosine Diphosphate
Communication
Pathology
Ligands

Keywords

  • Adenine and uridine nucleotides
  • Astrocytes
  • Calcium signaling
  • P2X receptors
  • P2Y receptors
  • UDP-glucose

ASJC Scopus subject areas

  • Immunology

Cite this

Fumagalli, M., Brambilla, R., D'Ambrosi, N., Volonté, C., Matteoli, M., Verderio, C., & Abbracchio, M. P. (2003). Nucleotide-mediated calcium signaling in rat cortical astrocytes: Role of P2X and P2Y receptors. GLIA, 43(3), 218-230. https://doi.org/10.1002/glia.10248

Nucleotide-mediated calcium signaling in rat cortical astrocytes : Role of P2X and P2Y receptors. / Fumagalli, Marta; Brambilla, Roberta; D'Ambrosi, Nadia; Volonté, Cinzia; Matteoli, Michela; Verderio, Claudia; Abbracchio, Maria P.

In: GLIA, Vol. 43, No. 3, 01.09.2003, p. 218-230.

Research output: Contribution to journalArticle

Fumagalli, M, Brambilla, R, D'Ambrosi, N, Volonté, C, Matteoli, M, Verderio, C & Abbracchio, MP 2003, 'Nucleotide-mediated calcium signaling in rat cortical astrocytes: Role of P2X and P2Y receptors', GLIA, vol. 43, no. 3, pp. 218-230. https://doi.org/10.1002/glia.10248
Fumagalli M, Brambilla R, D'Ambrosi N, Volonté C, Matteoli M, Verderio C et al. Nucleotide-mediated calcium signaling in rat cortical astrocytes: Role of P2X and P2Y receptors. GLIA. 2003 Sep 1;43(3):218-230. https://doi.org/10.1002/glia.10248
Fumagalli, Marta ; Brambilla, Roberta ; D'Ambrosi, Nadia ; Volonté, Cinzia ; Matteoli, Michela ; Verderio, Claudia ; Abbracchio, Maria P. / Nucleotide-mediated calcium signaling in rat cortical astrocytes : Role of P2X and P2Y receptors. In: GLIA. 2003 ; Vol. 43, No. 3. pp. 218-230.
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