Nucleotide dimers suppress HIV expression in vitro

M. Busso, A. M. Mian, E. F. Hahn, L. Resnick

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A series of nucleotide homo- and heterodimers [3'-azido-3'-deoxythymidilyl-(5',5')-2',3'-dideoxy-5'adenylic acid (AZT-P-ddA), [3'-azido-3'-deoxythymidilyl-(5',5')-2',3'-dideoxy;-5'adenylic acid, 2-cyanoethyl ester [AZT-P(CyE)-ddA], [3'-azido-3'-deoxythymidilyl-(5',5')-2',3'-dideoxy-5'-inosinic acid (AZT-P-ddI), and [3'-azido-3'-deoxythymidilyl-(5',5')-3'-azido-3'-deoxy-5'thymidilic acid (AZT-P-AZT)] were synthesized and compared with respect to their anti-HIV and cytotoxic properties to their component monomers in vitro. MT-2 cells were infected with HIV (TM) followed by the addition of drug. The dimers and their respective monomers inhibited HIV-induced syncytia formation, reverse transcriptase production, and the expression of HIV p24 antigen. However, on an equimolar basis, greater anti-HIV potency and enhanced cytotherapeutic indices were observed with the heterodimers when compared with their monomers. Nucleotide dimers, such as AZT-P-ddA, should be actively considered for further evaluation as anti-HIV agents.

Original languageEnglish
Pages (from-to)449-455
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume4
Issue number6
StatePublished - Dec 1 1988

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Nucleotides
HIV
Adenosine Monophosphate
HIV Core Protein p24
Anti-HIV Agents
RNA-Directed DNA Polymerase
Giant Cells
Esters
Acids
In Vitro Techniques
Pharmaceutical Preparations
scriptene

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Busso, M., Mian, A. M., Hahn, E. F., & Resnick, L. (1988). Nucleotide dimers suppress HIV expression in vitro. AIDS Research and Human Retroviruses, 4(6), 449-455.

Nucleotide dimers suppress HIV expression in vitro. / Busso, M.; Mian, A. M.; Hahn, E. F.; Resnick, L.

In: AIDS Research and Human Retroviruses, Vol. 4, No. 6, 01.12.1988, p. 449-455.

Research output: Contribution to journalArticle

Busso, M, Mian, AM, Hahn, EF & Resnick, L 1988, 'Nucleotide dimers suppress HIV expression in vitro', AIDS Research and Human Retroviruses, vol. 4, no. 6, pp. 449-455.
Busso M, Mian AM, Hahn EF, Resnick L. Nucleotide dimers suppress HIV expression in vitro. AIDS Research and Human Retroviruses. 1988 Dec 1;4(6):449-455.
Busso, M. ; Mian, A. M. ; Hahn, E. F. ; Resnick, L. / Nucleotide dimers suppress HIV expression in vitro. In: AIDS Research and Human Retroviruses. 1988 ; Vol. 4, No. 6. pp. 449-455.
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