Nuclear proteins involved in transcription of the human K5 keratin gene

Mamitaro Ohtsuki, Mariana Tomic-Canic, Irwin M. Freedberg, Miroslav Blumenberg, Ronald O. Perelman

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Keratin K5 is expressed in the basal layer of stratified epithelia in mammals and its synthesis is regulated by hormones and vitamins such as retinoic acid. The molecular mechanisms that regulate K5 expression are not known. To initiate analysis of the protein factors that interact with the human K5 keratin gene upstream region, we have used gel-retardation and DNA-mediated cell-transfection assays. We found five DNA sites that specifically bind nuclear proteins. DNA-protein interactions at two of the sites apparently increase transcription levels, at one decrease it. The importance of the remaining two sites is, at present, unclear. In addition, the location of the retinoic acid and thyroid hormone nuclear receptor action site has been determined, and we suggest that it involves a cluster of five sites similar to the consensus recognition elements. The complex constellation of protein binding sites upstream from the K5 gene probably reflects the complex regulatory circuits that govern the expression of the K5 keratin in mammalian tissues.

Original languageEnglish (US)
Pages (from-to)206-215
Number of pages10
JournalJournal of Investigative Dermatology
Volume99
Issue number2
DOIs
StatePublished - Aug 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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