Nuclear protein synthesis: A re-evaluation

Lubov Nathanson, Tianli Xia, Murray P Deutscher

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

It has been reported that nuclei from HeLa cells are responsible for -10%-15% of total cellular protein synthesis. We show here that isolated Chinese hamster ovary (CHO) and HeLa cell nuclei are essentially inactive for translation, and that the earlier results were most likely due to cytoplasmic contamination. Moreover, we suggest that the nascent polypeptides observed in nuclei of permeabilized cells may have been due to "overpermeabilization" and consequent damage to the cells. Based on this information, we conclude that nuclear protein synthesis, if it exists, is limited to less than 1% of that in cells.

Original languageEnglish
Pages (from-to)9-13
Number of pages5
JournalRNA
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2003

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Nuclear Proteins
Cell Nucleus
HeLa Cells
Cricetulus
Ovary
Peptides
Proteins

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Nathanson, L., Xia, T., & Deutscher, M. P. (2003). Nuclear protein synthesis: A re-evaluation. RNA, 9(1), 9-13. https://doi.org/10.1261/rna.2990203

Nuclear protein synthesis : A re-evaluation. / Nathanson, Lubov; Xia, Tianli; Deutscher, Murray P.

In: RNA, Vol. 9, No. 1, 01.01.2003, p. 9-13.

Research output: Contribution to journalArticle

Nathanson, L, Xia, T & Deutscher, MP 2003, 'Nuclear protein synthesis: A re-evaluation', RNA, vol. 9, no. 1, pp. 9-13. https://doi.org/10.1261/rna.2990203
Nathanson L, Xia T, Deutscher MP. Nuclear protein synthesis: A re-evaluation. RNA. 2003 Jan 1;9(1):9-13. https://doi.org/10.1261/rna.2990203
Nathanson, Lubov ; Xia, Tianli ; Deutscher, Murray P. / Nuclear protein synthesis : A re-evaluation. In: RNA. 2003 ; Vol. 9, No. 1. pp. 9-13.
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