Nuclear protein synthesis: A re-evaluation

Lubov Nathanson, Tianli Xia, Murray P. Deutscher

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

It has been reported that nuclei from HeLa cells are responsible for -10%-15% of total cellular protein synthesis. We show here that isolated Chinese hamster ovary (CHO) and HeLa cell nuclei are essentially inactive for translation, and that the earlier results were most likely due to cytoplasmic contamination. Moreover, we suggest that the nascent polypeptides observed in nuclei of permeabilized cells may have been due to "overpermeabilization" and consequent damage to the cells. Based on this information, we conclude that nuclear protein synthesis, if it exists, is limited to less than 1% of that in cells.

Original languageEnglish (US)
Pages (from-to)9-13
Number of pages5
JournalRNA
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2003

ASJC Scopus subject areas

  • Molecular Biology

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