TY - JOUR
T1 - Nuclear disintegration of target cells by killer B lymphocytes from tumor-bearing mice
AU - Lopez, D. M.
AU - Blomberg, B. B.
AU - Padmanabhan, R. R.
AU - Bourguignon, L. Y.W.
PY - 1989
Y1 - 1989
N2 - Normal murine B lymphocytes are not known to be effectors of the Fc receptor-mediated, antibody-dependent cellular cytotoxicity (ADCC). In contrast, we report here that highly purified splenic B cells from mammary tumor-bearing mice develop the potential of lysing antibody-coated target cells. These lymphocytes are characterized by being G-10 nonadherent, nylon wood adherent, sIg+, FcR+, Thy 1.2-, asialo GM1-, and the immunoglobulin heavy-chain genes of both chromosomes are rearranged. The lytic reaction is characterized by a noninterdigitating binding and by the appearance of endocytotic vescicles in the target cells. Nuclear disintegration occurs 18 h after initial effector-target cell conjugate formation. At such time, only minor cytoplasmic membrane alterations are evident. The emergence of killer B cells in tumor-bearing hosts indicates that all lymphoreticular cell types bearing Fc receptors are capable of mediating ADCC.
AB - Normal murine B lymphocytes are not known to be effectors of the Fc receptor-mediated, antibody-dependent cellular cytotoxicity (ADCC). In contrast, we report here that highly purified splenic B cells from mammary tumor-bearing mice develop the potential of lysing antibody-coated target cells. These lymphocytes are characterized by being G-10 nonadherent, nylon wood adherent, sIg+, FcR+, Thy 1.2-, asialo GM1-, and the immunoglobulin heavy-chain genes of both chromosomes are rearranged. The lytic reaction is characterized by a noninterdigitating binding and by the appearance of endocytotic vescicles in the target cells. Nuclear disintegration occurs 18 h after initial effector-target cell conjugate formation. At such time, only minor cytoplasmic membrane alterations are evident. The emergence of killer B cells in tumor-bearing hosts indicates that all lymphoreticular cell types bearing Fc receptors are capable of mediating ADCC.
UR - http://www.scopus.com/inward/record.url?scp=0024595601&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024595601&partnerID=8YFLogxK
U2 - 10.1096/fasebj.3.1.2783411
DO - 10.1096/fasebj.3.1.2783411
M3 - Article
C2 - 2783411
AN - SCOPUS:0024595601
VL - 3
SP - 37
EP - 43
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 1
ER -