Nuclear disintegration of target cells by killer B lymphocytes from tumor-bearing mice

D. M. Lopez, B. B. Blomberg, R. R. Padmanabhan, L. Y.W. Bourguignon

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Normal murine B lymphocytes are not known to be effectors of the Fc receptor-mediated, antibody-dependent cellular cytotoxicity (ADCC). In contrast, we report here that highly purified splenic B cells from mammary tumor-bearing mice develop the potential of lysing antibody-coated target cells. These lymphocytes are characterized by being G-10 nonadherent, nylon wood adherent, sIg+, FcR+, Thy 1.2-, asialo GM1-, and the immunoglobulin heavy-chain genes of both chromosomes are rearranged. The lytic reaction is characterized by a noninterdigitating binding and by the appearance of endocytotic vescicles in the target cells. Nuclear disintegration occurs 18 h after initial effector-target cell conjugate formation. At such time, only minor cytoplasmic membrane alterations are evident. The emergence of killer B cells in tumor-bearing hosts indicates that all lymphoreticular cell types bearing Fc receptors are capable of mediating ADCC.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalFASEB Journal
Volume3
Issue number1
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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