Nuclear disintegration of target cells by killer B lymphocytes from tumor-bearing mice

Diana M Lopez, Bonnie B Blomberg, R. R. Padmanabhan, L. Y W Bourguignon

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Normal murine B lymphocytes are not known to be effectors of the Fc receptor-mediated, antibody-dependent cellular cytotoxicity (ADCC). In contrast, we report here that highly purified splenic B cells from mammary tumor-bearing mice develop the potential of lysing antibody-coated target cells. These lymphocytes are characterized by being G-10 nonadherent, nylon wood adherent, sIg+, FcR+, Thy 1.2-, asialo GM1-, and the immunoglobulin heavy-chain genes of both chromosomes are rearranged. The lytic reaction is characterized by a noninterdigitating binding and by the appearance of endocytotic vescicles in the target cells. Nuclear disintegration occurs 18 h after initial effector-target cell conjugate formation. At such time, only minor cytoplasmic membrane alterations are evident. The emergence of killer B cells in tumor-bearing hosts indicates that all lymphoreticular cell types bearing Fc receptors are capable of mediating ADCC.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalFASEB Journal
Volume3
Issue number1
StatePublished - Jan 1 1989

Fingerprint

Bearings (structural)
Lymphocytes
Disintegration
B-lymphocytes
Tumors
B-Lymphocytes
Fc Receptors
Cytotoxicity
neoplasms
Antibodies
mice
Cells
antibodies
cytotoxicity
Neoplasms
Immunoglobulin Heavy Chains
Nylons
cells
immunoglobulin heavy chains
Chromosomes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Nuclear disintegration of target cells by killer B lymphocytes from tumor-bearing mice. / Lopez, Diana M; Blomberg, Bonnie B; Padmanabhan, R. R.; Bourguignon, L. Y W.

In: FASEB Journal, Vol. 3, No. 1, 01.01.1989, p. 37-43.

Research output: Contribution to journalArticle

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