NT4/5 mutant mice have deficiency in gustatory papillae and taste bud formation

Daniel J. Liebl, Joseph Pascal Mbiene, Luis F. Parada

Research output: Contribution to journalArticle

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Abstract

Neurotrophins are key determinants for controlling the survival of peripheral neurons during development. Brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT4/5) exert their action through a common trkB receptor but independently support gustatory sensory neurons. To assess the role of NT4/5 during development, we examined the postnatal development and maintenance of fungiform taste buds in mice carrying a deletion of NT4/5. The absence of NT4/5 results in embryonic deficits in gustatory innervation and a reduced number of fungiform papillae at birth. No degenerative deficits of fungiform papillae were observed for the first 3 weeks of postnatal development. However, these remaining fungiform papillae were smaller in appearance and many did not contain taste pores. By postnatal day 60, there was 63% decrease in the number of fungiform papillae, and remaining papillae were smaller in size or modified into filiform-like spines. These papillae had either no taste bud or a taste bud with a reduced number of taste cells compared to controls. These findings demonstrate that the NT4/5 gene functions in the maintenance of fungiform gustatory papillae and raises the possibility for an earlier role in development.

Original languageEnglish (US)
Pages (from-to)378-389
Number of pages12
JournalDevelopmental Biology
Volume213
Issue number2
DOIs
StatePublished - Sep 15 1999

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ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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