NT-3 and/or BDNF therapy prevents loss of auditory neurons following loss of hair cells

Hinrich Staecker, Richard Kopke, Brigitte Malgrange, Philippe Lefebvre, Thomas R. Van De Water

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237 Scopus citations

Abstract

DESTRUCTION of auditory hair cells results in a subsequent loss of auditory neurons. In situ hybridization and neuronal cell culture studies as well as analyses of the inner ears of neurotrophin and neurotrophin receptor gene knockout mice have shown that NT-3 and BDNF mediate both the development and survival of auditory neurons. In this study guinea pigs were exposed to the ototoxic combination of an aminoglycoside antibiotic and a loop diuretic and then received 8 weeks of intracochlear infusion of either NT-3, BDNF or NT-3 + BDNF to determine whether site-specific application of these neurotrophins could prevent the loss of auditory neurons that follows a loss of auditory hair cells. Infusion of either NT-3 or NT-3 + BDNF into the scala tympani resulted in a >90% survival of auditory neurons while BDNF infusion yielded a 78% survival rate, compared with a 14-24% neuronal survival rate in untreated ototoxin-exposed cochleae. These results show that loss of auditory neurons that occurs subsequent to a loss of auditory hair cells can be prevented by in vivo neurotrophin therapy with either NT-3 or BDNF.

Original languageEnglish (US)
Pages (from-to)889-894
Number of pages6
JournalNeuroreport
Volume7
Issue number4
DOIs
StatePublished - Jan 1 1996

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Keywords

  • Auditory neurons
  • Growth factor therapy
  • Neuronal survival
  • Neurotrophins
  • Otoxicity
  • Trophic support

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Staecker, H., Kopke, R., Malgrange, B., Lefebvre, P., & Van De Water, T. R. (1996). NT-3 and/or BDNF therapy prevents loss of auditory neurons following loss of hair cells. Neuroreport, 7(4), 889-894. https://doi.org/10.1097/00001756-199603220-00011