NRA-2, a nicalin homolog, regulates neuronal death by controlling surface localization of toxic caenorhabditis elegans DEG/ENaC channels

Shaunak Kamat, Shrutika Yeola, Wenying Zhang, Laura Bianchi, Monica Driscoll

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Mechanisms that regulate plasma membrane expression of neuronally toxic DEG/ENaC channels are unclear., Results: Disruption of ER NRA-2, a Nicalin homolog, enhances C. Elegans MEC-10(d) DEG/ENaC neurotoxicity. Immunocytochemistry, TIRF imaging, and electrophysiological assays support that NRA-2 controls relative MEC-10(d) distribution between ER and cell surface to regulate channel activity levels., Conclusion: NRA-2 regulates surface expression of a mutant DEG/ENaC channel., Significance: NRA-2 Nicalin can modulate DEG/ENaC pathophysiology.

Original languageEnglish
Pages (from-to)11916-11926
Number of pages11
JournalJournal of Biological Chemistry
Volume289
Issue number17
DOIs
StatePublished - Apr 25 2014

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Poisons
Caenorhabditis elegans
Immunohistochemistry
Cell Membrane
Cell membranes
Assays
Imaging techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

NRA-2, a nicalin homolog, regulates neuronal death by controlling surface localization of toxic caenorhabditis elegans DEG/ENaC channels. / Kamat, Shaunak; Yeola, Shrutika; Zhang, Wenying; Bianchi, Laura; Driscoll, Monica.

In: Journal of Biological Chemistry, Vol. 289, No. 17, 25.04.2014, p. 11916-11926.

Research output: Contribution to journalArticle

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AU - Bianchi, Laura

AU - Driscoll, Monica

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