Novel technique for online characterization of cartilaginous tissue properties

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The goal of tissue engineering is to use substitutes to repair and restore organ function. Bioreactors are an indispensable tool for monitoring and controlling the unique environment for engineered constructs to grow. However, in order to determine the biochemical properties of engineered constructs, samples need to be destroyed. In this study, we developed a novel technique to nondestructively online-characterize the water content and fixed charge density of cartilaginous tissues. A new technique was developed to determine the tissue mechano-electrochemical properties nondestructively. Bovine knee articular cartilage and lumbar annulus fibrosus were used in this study to demonstrate that this technique could be used on different types of tissue. The results show that our newly developed method is capable of precisely predicting the water volume fraction (less than 3 disparity) and fixed charge density (less than 16.7 disparity) within cartilaginous tissues. This novel technique will help to design a new generation of bioreactors which are able to actively determine the essential properties of the engineered constructs, as well as regulate the local environment to achieve the optimal conditions for cultivating constructs.

Original languageEnglish
Article number094504
JournalJournal of Biomechanical Engineering
Volume133
Issue number9
DOIs
StatePublished - Oct 28 2011

Fingerprint

Tissue
Bioreactors
Charge density
Water
Cartilage
Articular Cartilage
Tissue Engineering
Electrochemical properties
Tissue engineering
Water content
Volume fraction
Knee
Repair
Monitoring
Annulus Fibrosus

Keywords

  • Annulus fibrosus
  • Bioreactor
  • cartilage
  • Fixed charge density
  • Tissue engineering
  • Water content

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physiology (medical)

Cite this

Novel technique for online characterization of cartilaginous tissue properties. / Yuan, Tai Yi; Huang, Chun-Yuh; Gu, Weiyong.

In: Journal of Biomechanical Engineering, Vol. 133, No. 9, 094504, 28.10.2011.

Research output: Contribution to journalArticle

@article{e3c7487771ff4063a03ece82f3fa33d2,
title = "Novel technique for online characterization of cartilaginous tissue properties",
abstract = "The goal of tissue engineering is to use substitutes to repair and restore organ function. Bioreactors are an indispensable tool for monitoring and controlling the unique environment for engineered constructs to grow. However, in order to determine the biochemical properties of engineered constructs, samples need to be destroyed. In this study, we developed a novel technique to nondestructively online-characterize the water content and fixed charge density of cartilaginous tissues. A new technique was developed to determine the tissue mechano-electrochemical properties nondestructively. Bovine knee articular cartilage and lumbar annulus fibrosus were used in this study to demonstrate that this technique could be used on different types of tissue. The results show that our newly developed method is capable of precisely predicting the water volume fraction (less than 3 disparity) and fixed charge density (less than 16.7 disparity) within cartilaginous tissues. This novel technique will help to design a new generation of bioreactors which are able to actively determine the essential properties of the engineered constructs, as well as regulate the local environment to achieve the optimal conditions for cultivating constructs.",
keywords = "Annulus fibrosus, Bioreactor, cartilage, Fixed charge density, Tissue engineering, Water content",
author = "Yuan, {Tai Yi} and Chun-Yuh Huang and Weiyong Gu",
year = "2011",
month = "10",
day = "28",
doi = "10.1115/1.4004920",
language = "English",
volume = "133",
journal = "Journal of Biomechanical Engineering",
issn = "0148-0731",
publisher = "American Society of Mechanical Engineers(ASME)",
number = "9",

}

TY - JOUR

T1 - Novel technique for online characterization of cartilaginous tissue properties

AU - Yuan, Tai Yi

AU - Huang, Chun-Yuh

AU - Gu, Weiyong

PY - 2011/10/28

Y1 - 2011/10/28

N2 - The goal of tissue engineering is to use substitutes to repair and restore organ function. Bioreactors are an indispensable tool for monitoring and controlling the unique environment for engineered constructs to grow. However, in order to determine the biochemical properties of engineered constructs, samples need to be destroyed. In this study, we developed a novel technique to nondestructively online-characterize the water content and fixed charge density of cartilaginous tissues. A new technique was developed to determine the tissue mechano-electrochemical properties nondestructively. Bovine knee articular cartilage and lumbar annulus fibrosus were used in this study to demonstrate that this technique could be used on different types of tissue. The results show that our newly developed method is capable of precisely predicting the water volume fraction (less than 3 disparity) and fixed charge density (less than 16.7 disparity) within cartilaginous tissues. This novel technique will help to design a new generation of bioreactors which are able to actively determine the essential properties of the engineered constructs, as well as regulate the local environment to achieve the optimal conditions for cultivating constructs.

AB - The goal of tissue engineering is to use substitutes to repair and restore organ function. Bioreactors are an indispensable tool for monitoring and controlling the unique environment for engineered constructs to grow. However, in order to determine the biochemical properties of engineered constructs, samples need to be destroyed. In this study, we developed a novel technique to nondestructively online-characterize the water content and fixed charge density of cartilaginous tissues. A new technique was developed to determine the tissue mechano-electrochemical properties nondestructively. Bovine knee articular cartilage and lumbar annulus fibrosus were used in this study to demonstrate that this technique could be used on different types of tissue. The results show that our newly developed method is capable of precisely predicting the water volume fraction (less than 3 disparity) and fixed charge density (less than 16.7 disparity) within cartilaginous tissues. This novel technique will help to design a new generation of bioreactors which are able to actively determine the essential properties of the engineered constructs, as well as regulate the local environment to achieve the optimal conditions for cultivating constructs.

KW - Annulus fibrosus

KW - Bioreactor

KW - cartilage

KW - Fixed charge density

KW - Tissue engineering

KW - Water content

UR - http://www.scopus.com/inward/record.url?scp=80054847510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054847510&partnerID=8YFLogxK

U2 - 10.1115/1.4004920

DO - 10.1115/1.4004920

M3 - Article

C2 - 22010750

AN - SCOPUS:80054847510

VL - 133

JO - Journal of Biomechanical Engineering

JF - Journal of Biomechanical Engineering

SN - 0148-0731

IS - 9

M1 - 094504

ER -