Novel synapses compensate for a neuron ablated in embryos

B. K. Modney, Kenneth J Muller

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In leeches, as well as mammals, neuronal death in adults produces lasting deficits, whereas the embryonic nervous system is believed to be more plastic. Killing the single S interneuron in an adult leech ganglion permanently interrupts the chain of S cells linked by electrical synapses along the entire animal. Axons that synapsed with the ablated neuron do not change length in response to cell ablation, but they will grow if another axon of the same neuron is injured. In the present experiments, the S cell and surrounding cells in one ganglion were ablated with a fine pin during embryogenesis (day 8-11). Effects were evaluated 1-4 months later. Cell-specific monoclonal antibody confirmed S cell deletions. Intracellular injection of horseradish peroxidase and 6-carboxyfluorescein dye showed that intact S cells' axons projected twice their usual length into the lesioned ganglion and formed electrical synapses with homologues of their usual synaptic targets. Conduction was often restored by these connections, which replaced those of the deleted S cell. Therefore, in both adults and embryos, growing S interneurons respond to loss of a target by greater growth. However, only on the small scale of the embryo is growth sufficient to reach suitable targets.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
JournalProceedings of the Royal Society B: Biological Sciences
Volume257
Issue number1350
StatePublished - Oct 10 1994
Externally publishedYes

Fingerprint

synapse
Synapses
Neurons
embryo
leech
embryo (animal)
Embryonic Structures
neurons
Mammals
Electrical Synapses
axons
Neurology
Ganglia
cells
Horseradish Peroxidase
nervous system
Ablation
Axons
Leeches
ablation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Novel synapses compensate for a neuron ablated in embryos. / Modney, B. K.; Muller, Kenneth J.

In: Proceedings of the Royal Society B: Biological Sciences, Vol. 257, No. 1350, 10.10.1994, p. 263-269.

Research output: Contribution to journalArticle

@article{2545d6997b5c433ea218d69a6b8b7653,
title = "Novel synapses compensate for a neuron ablated in embryos",
abstract = "In leeches, as well as mammals, neuronal death in adults produces lasting deficits, whereas the embryonic nervous system is believed to be more plastic. Killing the single S interneuron in an adult leech ganglion permanently interrupts the chain of S cells linked by electrical synapses along the entire animal. Axons that synapsed with the ablated neuron do not change length in response to cell ablation, but they will grow if another axon of the same neuron is injured. In the present experiments, the S cell and surrounding cells in one ganglion were ablated with a fine pin during embryogenesis (day 8-11). Effects were evaluated 1-4 months later. Cell-specific monoclonal antibody confirmed S cell deletions. Intracellular injection of horseradish peroxidase and 6-carboxyfluorescein dye showed that intact S cells' axons projected twice their usual length into the lesioned ganglion and formed electrical synapses with homologues of their usual synaptic targets. Conduction was often restored by these connections, which replaced those of the deleted S cell. Therefore, in both adults and embryos, growing S interneurons respond to loss of a target by greater growth. However, only on the small scale of the embryo is growth sufficient to reach suitable targets.",
author = "Modney, {B. K.} and Muller, {Kenneth J}",
year = "1994",
month = "10",
day = "10",
language = "English",
volume = "257",
pages = "263--269",
journal = "Proceedings of the Royal Society B: Biological Sciences",
issn = "0800-4622",
publisher = "Royal Society of London",
number = "1350",

}

TY - JOUR

T1 - Novel synapses compensate for a neuron ablated in embryos

AU - Modney, B. K.

AU - Muller, Kenneth J

PY - 1994/10/10

Y1 - 1994/10/10

N2 - In leeches, as well as mammals, neuronal death in adults produces lasting deficits, whereas the embryonic nervous system is believed to be more plastic. Killing the single S interneuron in an adult leech ganglion permanently interrupts the chain of S cells linked by electrical synapses along the entire animal. Axons that synapsed with the ablated neuron do not change length in response to cell ablation, but they will grow if another axon of the same neuron is injured. In the present experiments, the S cell and surrounding cells in one ganglion were ablated with a fine pin during embryogenesis (day 8-11). Effects were evaluated 1-4 months later. Cell-specific monoclonal antibody confirmed S cell deletions. Intracellular injection of horseradish peroxidase and 6-carboxyfluorescein dye showed that intact S cells' axons projected twice their usual length into the lesioned ganglion and formed electrical synapses with homologues of their usual synaptic targets. Conduction was often restored by these connections, which replaced those of the deleted S cell. Therefore, in both adults and embryos, growing S interneurons respond to loss of a target by greater growth. However, only on the small scale of the embryo is growth sufficient to reach suitable targets.

AB - In leeches, as well as mammals, neuronal death in adults produces lasting deficits, whereas the embryonic nervous system is believed to be more plastic. Killing the single S interneuron in an adult leech ganglion permanently interrupts the chain of S cells linked by electrical synapses along the entire animal. Axons that synapsed with the ablated neuron do not change length in response to cell ablation, but they will grow if another axon of the same neuron is injured. In the present experiments, the S cell and surrounding cells in one ganglion were ablated with a fine pin during embryogenesis (day 8-11). Effects were evaluated 1-4 months later. Cell-specific monoclonal antibody confirmed S cell deletions. Intracellular injection of horseradish peroxidase and 6-carboxyfluorescein dye showed that intact S cells' axons projected twice their usual length into the lesioned ganglion and formed electrical synapses with homologues of their usual synaptic targets. Conduction was often restored by these connections, which replaced those of the deleted S cell. Therefore, in both adults and embryos, growing S interneurons respond to loss of a target by greater growth. However, only on the small scale of the embryo is growth sufficient to reach suitable targets.

UR - http://www.scopus.com/inward/record.url?scp=0028021998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028021998&partnerID=8YFLogxK

M3 - Article

C2 - 7991636

AN - SCOPUS:0028021998

VL - 257

SP - 263

EP - 269

JO - Proceedings of the Royal Society B: Biological Sciences

JF - Proceedings of the Royal Society B: Biological Sciences

SN - 0800-4622

IS - 1350

ER -