Novel pluripotent adult stem cell source for neurogenesis

Veronica R. Fortino, Devon Pawley, Daniel Pelaez, Herman S Cheung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel pluripotent stem cell population discovered by our laboratory, periodontal ligament stem cells (PDLSCs) are a viable source for regenerative medicine. We have demonstrated that these cells form all three germ layers in-vivo, and hypothesized that PDLSCs would be an ideal cell source for neurogenesis in-vitro. In our study, we evaluate the potential of Connexin 43+ PDLSCs to differentiate into a neural lineage. Control Connexin 43+ PDLSCs were cultured in complete culture media. Neuro-induced Connexin 43+ PDLSCs were grown in a neurogenic cocktail media. Media was changed every three days for a total of 6 days of treatment. Following treatment, cell morphology, gene expression, and immunohistochemistry were evaluated. Gene expression data was analyzed using a two-tailed t-test, with p<0.05 considered significant. Morphological differences were evident in neuroinduced Connexin 43+ PDLSCs, along with a significant increase in gene expression of neuro filament medium over control samples. Immunohistochemical staining in neuroinduced Connexin 43+ PDLSCs demonstrated neurite-like processes and synaptophysin not found in control samples. Conclusion: Our neuro-induction protocol successfully induces Connexin 43+ PDLSCs to a neural lineage.

Original languageEnglish
Title of host publicationProceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013
Pages15-16
Number of pages2
DOIs
StatePublished - Aug 5 2013
Event29th Southern Biomedical Engineering Conference, SBEC 2013 - Miami, FL, United States
Duration: May 3 2013May 5 2013

Other

Other29th Southern Biomedical Engineering Conference, SBEC 2013
CountryUnited States
CityMiami, FL
Period5/3/135/5/13

Fingerprint

Stem cells
Ligaments
Gene expression
Cell culture

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Fortino, V. R., Pawley, D., Pelaez, D., & Cheung, H. S. (2013). Novel pluripotent adult stem cell source for neurogenesis. In Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013 (pp. 15-16). [6525653] https://doi.org/10.1109/SBEC.2013.16

Novel pluripotent adult stem cell source for neurogenesis. / Fortino, Veronica R.; Pawley, Devon; Pelaez, Daniel; Cheung, Herman S.

Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013. 2013. p. 15-16 6525653.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fortino, VR, Pawley, D, Pelaez, D & Cheung, HS 2013, Novel pluripotent adult stem cell source for neurogenesis. in Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013., 6525653, pp. 15-16, 29th Southern Biomedical Engineering Conference, SBEC 2013, Miami, FL, United States, 5/3/13. https://doi.org/10.1109/SBEC.2013.16
Fortino VR, Pawley D, Pelaez D, Cheung HS. Novel pluripotent adult stem cell source for neurogenesis. In Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013. 2013. p. 15-16. 6525653 https://doi.org/10.1109/SBEC.2013.16
Fortino, Veronica R. ; Pawley, Devon ; Pelaez, Daniel ; Cheung, Herman S. / Novel pluripotent adult stem cell source for neurogenesis. Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013. 2013. pp. 15-16
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