Abstract
A novel pluripotent stem cell population discovered by our laboratory, periodontal ligament stem cells (PDLSCs) are a viable source for regenerative medicine. We have demonstrated that these cells form all three germ layers in-vivo, and hypothesized that PDLSCs would be an ideal cell source for neurogenesis in-vitro. In our study, we evaluate the potential of Connexin 43+ PDLSCs to differentiate into a neural lineage. Control Connexin 43+ PDLSCs were cultured in complete culture media. Neuro-induced Connexin 43+ PDLSCs were grown in a neurogenic cocktail media. Media was changed every three days for a total of 6 days of treatment. Following treatment, cell morphology, gene expression, and immunohistochemistry were evaluated. Gene expression data was analyzed using a two-tailed t-test, with p<0.05 considered significant. Morphological differences were evident in neuroinduced Connexin 43+ PDLSCs, along with a significant increase in gene expression of neuro filament medium over control samples. Immunohistochemical staining in neuroinduced Connexin 43+ PDLSCs demonstrated neurite-like processes and synaptophysin not found in control samples. Conclusion: Our neuro-induction protocol successfully induces Connexin 43+ PDLSCs to a neural lineage.
| Original language | English |
|---|---|
| Title of host publication | Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013 |
| Pages | 15-16 |
| Number of pages | 2 |
| DOIs | |
| State | Published - Aug 5 2013 |
| Event | 29th Southern Biomedical Engineering Conference, SBEC 2013 - Miami, FL, United States Duration: May 3 2013 → May 5 2013 |
Other
| Other | 29th Southern Biomedical Engineering Conference, SBEC 2013 |
|---|---|
| Country | United States |
| City | Miami, FL |
| Period | 5/3/13 → 5/5/13 |
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ASJC Scopus subject areas
- Biomedical Engineering
Cite this
Novel pluripotent adult stem cell source for neurogenesis. / Fortino, Veronica R.; Pawley, Devon; Pelaez, Daniel; Cheung, Herman S.
Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013. 2013. p. 15-16 6525653.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Novel pluripotent adult stem cell source for neurogenesis
AU - Fortino, Veronica R.
AU - Pawley, Devon
AU - Pelaez, Daniel
AU - Cheung, Herman S
PY - 2013/8/5
Y1 - 2013/8/5
N2 - A novel pluripotent stem cell population discovered by our laboratory, periodontal ligament stem cells (PDLSCs) are a viable source for regenerative medicine. We have demonstrated that these cells form all three germ layers in-vivo, and hypothesized that PDLSCs would be an ideal cell source for neurogenesis in-vitro. In our study, we evaluate the potential of Connexin 43+ PDLSCs to differentiate into a neural lineage. Control Connexin 43+ PDLSCs were cultured in complete culture media. Neuro-induced Connexin 43+ PDLSCs were grown in a neurogenic cocktail media. Media was changed every three days for a total of 6 days of treatment. Following treatment, cell morphology, gene expression, and immunohistochemistry were evaluated. Gene expression data was analyzed using a two-tailed t-test, with p<0.05 considered significant. Morphological differences were evident in neuroinduced Connexin 43+ PDLSCs, along with a significant increase in gene expression of neuro filament medium over control samples. Immunohistochemical staining in neuroinduced Connexin 43+ PDLSCs demonstrated neurite-like processes and synaptophysin not found in control samples. Conclusion: Our neuro-induction protocol successfully induces Connexin 43+ PDLSCs to a neural lineage.
AB - A novel pluripotent stem cell population discovered by our laboratory, periodontal ligament stem cells (PDLSCs) are a viable source for regenerative medicine. We have demonstrated that these cells form all three germ layers in-vivo, and hypothesized that PDLSCs would be an ideal cell source for neurogenesis in-vitro. In our study, we evaluate the potential of Connexin 43+ PDLSCs to differentiate into a neural lineage. Control Connexin 43+ PDLSCs were cultured in complete culture media. Neuro-induced Connexin 43+ PDLSCs were grown in a neurogenic cocktail media. Media was changed every three days for a total of 6 days of treatment. Following treatment, cell morphology, gene expression, and immunohistochemistry were evaluated. Gene expression data was analyzed using a two-tailed t-test, with p<0.05 considered significant. Morphological differences were evident in neuroinduced Connexin 43+ PDLSCs, along with a significant increase in gene expression of neuro filament medium over control samples. Immunohistochemical staining in neuroinduced Connexin 43+ PDLSCs demonstrated neurite-like processes and synaptophysin not found in control samples. Conclusion: Our neuro-induction protocol successfully induces Connexin 43+ PDLSCs to a neural lineage.
UR - http://www.scopus.com/inward/record.url?scp=84880907248&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880907248&partnerID=8YFLogxK
U2 - 10.1109/SBEC.2013.16
DO - 10.1109/SBEC.2013.16
M3 - Conference contribution
SN - 9780769550329
SP - 15
EP - 16
BT - Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013
ER -