Novel group of virulence activators within the AraC family that are not restricted to upstream binding sites

George Munson, L. G. Holcomb, J. R. Scott

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Several regulators within the AraC family control the expression of genes known or thought to be required for virulence of bacterial pathogens. One of these, Rns, activates transcription from an unprecedented variety of binding-site locations. Although nearly all prokaryotic activators bind within a small region upstream and adjacent to the promoter that they regulate, Rns does not bind within this region to activate its own promoter, Prns. Instead, to activate Prns, Rns requires one binding site 224.5 bp upstream and one downstream of the transcription start site. We show in this study that several other AraC family activators recognize the same binding sites as Rns and share with it the ability to utilize a downstream binding site. Like Rns, other members of this group of activators positively regulate the expression of virulence factors in pathogenic bacteria. These regulators are also able to activate transcription from promoter-proximal upstream binding sites since they are able to substitute for Rns at Pcoo, a promoter with only upstream binding sites. Thus, Rns is the prototype for a group of regulators, which include CfaR, VirF, AggR, and CsvR and which activate transcription from locations that are more diverse than those of any other known activator.

Original languageEnglish
Pages (from-to)186-193
Number of pages8
JournalInfection and Immunity
Volume69
Issue number1
DOIs
StatePublished - Jan 16 2001
Externally publishedYes

Fingerprint

Virulence
Binding Sites
Transcription Initiation Site
Virulence Factors
Bacteria
Gene Expression

ASJC Scopus subject areas

  • Immunology

Cite this

Novel group of virulence activators within the AraC family that are not restricted to upstream binding sites. / Munson, George; Holcomb, L. G.; Scott, J. R.

In: Infection and Immunity, Vol. 69, No. 1, 16.01.2001, p. 186-193.

Research output: Contribution to journalArticle

@article{f2112f09faef4d1691769213b82ca171,
title = "Novel group of virulence activators within the AraC family that are not restricted to upstream binding sites",
abstract = "Several regulators within the AraC family control the expression of genes known or thought to be required for virulence of bacterial pathogens. One of these, Rns, activates transcription from an unprecedented variety of binding-site locations. Although nearly all prokaryotic activators bind within a small region upstream and adjacent to the promoter that they regulate, Rns does not bind within this region to activate its own promoter, Prns. Instead, to activate Prns, Rns requires one binding site 224.5 bp upstream and one downstream of the transcription start site. We show in this study that several other AraC family activators recognize the same binding sites as Rns and share with it the ability to utilize a downstream binding site. Like Rns, other members of this group of activators positively regulate the expression of virulence factors in pathogenic bacteria. These regulators are also able to activate transcription from promoter-proximal upstream binding sites since they are able to substitute for Rns at Pcoo, a promoter with only upstream binding sites. Thus, Rns is the prototype for a group of regulators, which include CfaR, VirF, AggR, and CsvR and which activate transcription from locations that are more diverse than those of any other known activator.",
author = "George Munson and Holcomb, {L. G.} and Scott, {J. R.}",
year = "2001",
month = "1",
day = "16",
doi = "10.1128/IAI.69.1.186-193.2001",
language = "English",
volume = "69",
pages = "186--193",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Novel group of virulence activators within the AraC family that are not restricted to upstream binding sites

AU - Munson, George

AU - Holcomb, L. G.

AU - Scott, J. R.

PY - 2001/1/16

Y1 - 2001/1/16

N2 - Several regulators within the AraC family control the expression of genes known or thought to be required for virulence of bacterial pathogens. One of these, Rns, activates transcription from an unprecedented variety of binding-site locations. Although nearly all prokaryotic activators bind within a small region upstream and adjacent to the promoter that they regulate, Rns does not bind within this region to activate its own promoter, Prns. Instead, to activate Prns, Rns requires one binding site 224.5 bp upstream and one downstream of the transcription start site. We show in this study that several other AraC family activators recognize the same binding sites as Rns and share with it the ability to utilize a downstream binding site. Like Rns, other members of this group of activators positively regulate the expression of virulence factors in pathogenic bacteria. These regulators are also able to activate transcription from promoter-proximal upstream binding sites since they are able to substitute for Rns at Pcoo, a promoter with only upstream binding sites. Thus, Rns is the prototype for a group of regulators, which include CfaR, VirF, AggR, and CsvR and which activate transcription from locations that are more diverse than those of any other known activator.

AB - Several regulators within the AraC family control the expression of genes known or thought to be required for virulence of bacterial pathogens. One of these, Rns, activates transcription from an unprecedented variety of binding-site locations. Although nearly all prokaryotic activators bind within a small region upstream and adjacent to the promoter that they regulate, Rns does not bind within this region to activate its own promoter, Prns. Instead, to activate Prns, Rns requires one binding site 224.5 bp upstream and one downstream of the transcription start site. We show in this study that several other AraC family activators recognize the same binding sites as Rns and share with it the ability to utilize a downstream binding site. Like Rns, other members of this group of activators positively regulate the expression of virulence factors in pathogenic bacteria. These regulators are also able to activate transcription from promoter-proximal upstream binding sites since they are able to substitute for Rns at Pcoo, a promoter with only upstream binding sites. Thus, Rns is the prototype for a group of regulators, which include CfaR, VirF, AggR, and CsvR and which activate transcription from locations that are more diverse than those of any other known activator.

UR - http://www.scopus.com/inward/record.url?scp=0035159950&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035159950&partnerID=8YFLogxK

U2 - 10.1128/IAI.69.1.186-193.2001

DO - 10.1128/IAI.69.1.186-193.2001

M3 - Article

C2 - 11119505

AN - SCOPUS:0035159950

VL - 69

SP - 186

EP - 193

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 1

ER -