Novel experimental model for repeated forebrain ischemia-reperfusion

Lei Chen, Yu Shang, Eszter Sipos, Kathryn E. Kathryn, Yu Guoaiana, Michal Toborek

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Background and Purpose: The lack of reliable rodent model for repeated ischemia-reperfusion (I-R) hampers experimental research on stroke. Therefore, the objective of the present study was to develop a mouse model for repeated I-R cycles in a single animal on different days. Methods and Results: The right common carotid artery (CCA) was ligated and both vertebral arteries were coagulated. A customized vascular occluder, with its actuating tubing glued to a microport, was cuffed around the isolated left CCA and secured. Inflating of the occluder diaphragm via the microport restricted the blood flow via the left CCA and reduced the cerebral blood flow (CBF) by 75% in both hemispheres, while deflating allowed for the CBF restoration. Two minutes of forebrain ischemia followed by a 24 h reperfusion period was tolerated by animals for 5 cycles. Importantly, repeated 2 min I-R cycles attenuated the infarct volume induced by occlusion of the middle cerebral artery. Conclusions: The described model is a reliable method to induce transient I-R events in the forebrain. The model mimics transient ischemic attacks and allows for controlling the ischemic durations, intervals, and numbers of I-R cycles.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalJournal of Experimental Stroke and Translational Medicine
Volume5
Issue number1
DOIs
StatePublished - Jan 2012
Externally publishedYes

Keywords

  • Cerebral blood flow
  • Ischemia-reperfusion
  • Preconditioning
  • Stroke

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Clinical Neurology
  • Cognitive Neuroscience
  • Neurology

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