Novel conjugates of epitope fusion peptides with CpG-ODN display enhanced immunogenicity and HIV recognition

Pirouz Daftarian, Rahul Sharan, Wahajul Haq, Saima Ali, Jeff Longmate, John Termini, Don J. Diamond

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Vaccination strategies remain elusive that are effective against viral disease pathogens yet remain gentle enough for widespread human use. We developed a model system that relies on the recognition of specific T-cell epitopes from immunodominant antigens of HIV to explore single-stranded CpG-oligodeoxynucleotides (ODN) (CpG) as an adjuvant. We improved upon current strategies of utilizing CpG in combination with peptide vaccines by covalently modifying epitope fusion peptides with CpG motifs. Characterization of the immune recognition of DNA-peptide conjugates was carried out in a murine model of human HLA A2. Immunogenicity of DNA-peptide conjugates was superior in sensitivity to non-covalently linked mixtures of the same functional molecules as measured by peptide-mediated cytotoxicity and IFN-γ release, as well as protection against viral infection. Enhancement of sensitivity of immune recognition by covalent attachment of DNA to epitope peptides should be further evaluated as a novel prophylactic vaccine strategy for HIV infection and other infectious diseases.

Original languageEnglish (US)
Pages (from-to)3453-3468
Number of pages16
JournalVaccine
Volume23
Issue number26
DOIs
StatePublished - May 16 2005

Keywords

  • CpG
  • Epitope
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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    Daftarian, P., Sharan, R., Haq, W., Ali, S., Longmate, J., Termini, J., & Diamond, D. J. (2005). Novel conjugates of epitope fusion peptides with CpG-ODN display enhanced immunogenicity and HIV recognition. Vaccine, 23(26), 3453-3468. https://doi.org/10.1016/j.vaccine.2005.01.093