Notch3/Jagged1 circuitry reinforces notch signaling and sustains T-ALL

Maria Pelullo, Roberta Quaranta, Claudio Talora, Saula Checquolo, Samantha Cialfi, Maria Pia Felli, Geertruyte Kronnie, Chiara Borga, Zein Mersini Besharat, Rocco Palermo, Lucia Di Marcotullio, Anthony J. Capobianco, Alberto Gulino, Isabella Screpanti, Diana Bellavia

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Deregulated Notch signaling has been extensively linked to T-cell acute lymphoblastic leukemia (T-ALL). Here, we show a direct relationship between Notch3 receptor and Jagged1 ligand in human cell lines and in a mouse model of T-ALL. We provide evidence that Notch-specific ligand Jagged1 is a new Notch3 signaling target gene. This essential event justifies an aberrant Notch3/Jagged1 cis-expression inside the same cell. Moreover, we demonstrate in Notch3-IC- overexpressing T lymphoma cells that Jagged1 undergoes a raft-associated constitutive processing. The proteolytic cleavage allows the Jagged1 intracellulardomain toempower Notch signaling activity and to increase the transcriptional activation of Jagged1 itself (autocrine effect). On the other hand, the release of the soluble Jagged1 extracellular domain has a positive impact on activating Notch signaling in adjacent cells (paracrine effect), finally giving rise to a Notch3/Jagged1 auto-sustaining loop that supports the survival, proliferation, and invasion of lymphoma cells and contributes to the development and progression of Notch-dependent T-ALL. These observations are also supported by a study conducted on a cohort of patients in which Jagged1 expression is associated to adverse prognosis.

Original languageEnglish (US)
Pages (from-to)1007-1017
Number of pages11
JournalNeoplasia (United States)
Issue number12
StatePublished - 2014

ASJC Scopus subject areas

  • Cancer Research


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