Not all cytokine-producing CD8+ T cells suppress simian immunodeficiency virus replication

Won Chung, Wonhee Lee, John T. Loffredo, Benjamin Burwitz, Thomas C. Friedrich, Juan Pablo Giraldo Vela, Gnankang Napoe, Eva G. Rakasz, Nancy A. Wilson, David B. Allison, David Watkins

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Current assays of CD8+ T-lymphocyte function measure cytokine production rather than the ability of these lymphocytes to suppress viral replication. Here we show that CD8+ T-cell clones recognizing the same epitope vary enormously in the ability to suppress simian immunodeficiency virus SIVmac239 replication in an in vitro suppression assay. However, all Nef 165-173IW9- and Vif66-73HW8-speciflc clones from elite controllers effectively suppressed SIV replication. Interestingly, in vitro suppression efficacy was not always associated with the ability to produce gamma interferon, tumor necrosis factor alpha, or interleukin-2.

Original languageEnglish
Pages (from-to)1517-1523
Number of pages7
JournalJournal of Virology
Volume81
Issue number3
DOIs
StatePublished - Feb 1 2007
Externally publishedYes

Fingerprint

Simian immunodeficiency virus
Simian Immunodeficiency Virus
Virus Replication
virus replication
cytokines
Clone Cells
T-lymphocytes
clones
Cytokines
T-Lymphocytes
assays
interleukin-2
interferon-gamma
epitopes
tumor necrosis factor-alpha
Interferon-gamma
Interleukin-2
Epitopes
lymphocytes
Tumor Necrosis Factor-alpha

ASJC Scopus subject areas

  • Immunology

Cite this

Chung, W., Lee, W., Loffredo, J. T., Burwitz, B., Friedrich, T. C., Vela, J. P. G., ... Watkins, D. (2007). Not all cytokine-producing CD8+ T cells suppress simian immunodeficiency virus replication. Journal of Virology, 81(3), 1517-1523. https://doi.org/10.1128/JVI.01780-06

Not all cytokine-producing CD8+ T cells suppress simian immunodeficiency virus replication. / Chung, Won; Lee, Wonhee; Loffredo, John T.; Burwitz, Benjamin; Friedrich, Thomas C.; Vela, Juan Pablo Giraldo; Napoe, Gnankang; Rakasz, Eva G.; Wilson, Nancy A.; Allison, David B.; Watkins, David.

In: Journal of Virology, Vol. 81, No. 3, 01.02.2007, p. 1517-1523.

Research output: Contribution to journalArticle

Chung, W, Lee, W, Loffredo, JT, Burwitz, B, Friedrich, TC, Vela, JPG, Napoe, G, Rakasz, EG, Wilson, NA, Allison, DB & Watkins, D 2007, 'Not all cytokine-producing CD8+ T cells suppress simian immunodeficiency virus replication', Journal of Virology, vol. 81, no. 3, pp. 1517-1523. https://doi.org/10.1128/JVI.01780-06
Chung W, Lee W, Loffredo JT, Burwitz B, Friedrich TC, Vela JPG et al. Not all cytokine-producing CD8+ T cells suppress simian immunodeficiency virus replication. Journal of Virology. 2007 Feb 1;81(3):1517-1523. https://doi.org/10.1128/JVI.01780-06
Chung, Won ; Lee, Wonhee ; Loffredo, John T. ; Burwitz, Benjamin ; Friedrich, Thomas C. ; Vela, Juan Pablo Giraldo ; Napoe, Gnankang ; Rakasz, Eva G. ; Wilson, Nancy A. ; Allison, David B. ; Watkins, David. / Not all cytokine-producing CD8+ T cells suppress simian immunodeficiency virus replication. In: Journal of Virology. 2007 ; Vol. 81, No. 3. pp. 1517-1523.
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