Normal taste acceptance and preference of PANX1 knockout mice

Michael G. Tordoff; Tiffany R. Aleman; Hillary T. Ellis; Makoto Ohmoto; Ichiro Matsumoto; Val I. Shestopalov; Claire H. Mitchell; J. Kevin Foskett; Rachel L. Poole

doi:10.1093/chemse/bjv025

Normal taste acceptance and preference of PANX1 knockout mice

Michael G. Tordoff, Tiffany R. Aleman, Hillary T. Ellis, Makoto Ohmoto, Ichiro Matsumoto, Val I. Shestopalov, Claire H. Mitchell, J. Kevin Foskett, Rachel L. Poole

Ophthalmology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Taste compounds detected by G protein-coupled receptors on the apical surface of Type 2 taste cells initiate an intracellular molecular cascade culminating in the release of ATP. It has been suggested that this ATP release is accomplished by pannexin 1 (PANX1). However, we report here that PANX1 knockout mice do not differ from wild-type controls in response to representative taste solutions, measured using 5-s brief-access tests or 48-h two-bottle choice tests. This implies that PANX1 is unnecessary for taste detection and consequently that ATP release from Type 2 taste cells does not require PANX1.

Original language	English (US)
Pages (from-to)	453-459
Number of pages	7
Journal	Chemical senses
Volume	40
Issue number	7
DOIs	https://doi.org/10.1093/chemse/bjv025
State	Published - Sep 2015

Keywords

ATP release
CALHM1
Gustometer
Pannexin 1
Two-bottle choice test

ASJC Scopus subject areas

Physiology
Sensory Systems
Physiology (medical)
Behavioral Neuroscience

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10.1093/chemse/bjv025

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title = "Normal taste acceptance and preference of PANX1 knockout mice",

abstract = "Taste compounds detected by G protein-coupled receptors on the apical surface of Type 2 taste cells initiate an intracellular molecular cascade culminating in the release of ATP. It has been suggested that this ATP release is accomplished by pannexin 1 (PANX1). However, we report here that PANX1 knockout mice do not differ from wild-type controls in response to representative taste solutions, measured using 5-s brief-access tests or 48-h two-bottle choice tests. This implies that PANX1 is unnecessary for taste detection and consequently that ATP release from Type 2 taste cells does not require PANX1.",

keywords = "ATP release, CALHM1, Gustometer, Pannexin 1, Two-bottle choice test",

author = "Tordoff, {Michael G.} and Aleman, {Tiffany R.} and Ellis, {Hillary T.} and Makoto Ohmoto and Ichiro Matsumoto and Shestopalov, {Val I.} and Mitchell, {Claire H.} and Foskett, {J. Kevin} and Poole, {Rachel L.}",

note = "Funding Information: This work was supported by National Institutes of Health grant DK-46791 to M.G.T., DC-12538 to J.K.F., and EY021517 to V.I.S. Mouse gustometry was performed using equipment belonging to the Monell Phenotyping Core, which is supported, in part, by funding from the National Institute on Deafness and Other Communication Disorders P30 Core Grant DC011735. R.L.P. was supported by National Institute on Deafness and Other Communication Disorders Training Grant T32 DC000014. Publisher Copyright: {\textcopyright} The Author 2015.",

year = "2015",

month = sep,

doi = "10.1093/chemse/bjv025",

language = "English (US)",

volume = "40",

pages = "453--459",

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AU - Tordoff, Michael G.

AU - Aleman, Tiffany R.

AU - Ellis, Hillary T.

AU - Ohmoto, Makoto

AU - Matsumoto, Ichiro

AU - Shestopalov, Val I.

AU - Mitchell, Claire H.

AU - Foskett, J. Kevin

AU - Poole, Rachel L.

N1 - Funding Information: This work was supported by National Institutes of Health grant DK-46791 to M.G.T., DC-12538 to J.K.F., and EY021517 to V.I.S. Mouse gustometry was performed using equipment belonging to the Monell Phenotyping Core, which is supported, in part, by funding from the National Institute on Deafness and Other Communication Disorders P30 Core Grant DC011735. R.L.P. was supported by National Institute on Deafness and Other Communication Disorders Training Grant T32 DC000014. Publisher Copyright: © The Author 2015.

PY - 2015/9

Y1 - 2015/9

N2 - Taste compounds detected by G protein-coupled receptors on the apical surface of Type 2 taste cells initiate an intracellular molecular cascade culminating in the release of ATP. It has been suggested that this ATP release is accomplished by pannexin 1 (PANX1). However, we report here that PANX1 knockout mice do not differ from wild-type controls in response to representative taste solutions, measured using 5-s brief-access tests or 48-h two-bottle choice tests. This implies that PANX1 is unnecessary for taste detection and consequently that ATP release from Type 2 taste cells does not require PANX1.

AB - Taste compounds detected by G protein-coupled receptors on the apical surface of Type 2 taste cells initiate an intracellular molecular cascade culminating in the release of ATP. It has been suggested that this ATP release is accomplished by pannexin 1 (PANX1). However, we report here that PANX1 knockout mice do not differ from wild-type controls in response to representative taste solutions, measured using 5-s brief-access tests or 48-h two-bottle choice tests. This implies that PANX1 is unnecessary for taste detection and consequently that ATP release from Type 2 taste cells does not require PANX1.

KW - ATP release

KW - CALHM1

KW - Gustometer

KW - Pannexin 1

KW - Two-bottle choice test

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JO - Chemical Senses

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Normal taste acceptance and preference of PANX1 knockout mice

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Keywords

ASJC Scopus subject areas

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