Normal and neoplastic Schwann cells in fish - Isolation, culture and preparation for electron microscopy

Michael C. Schmale, Kenneth A. Gill, Saul M. Cacal

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Peripheral nerve sheath (PNS) neoplasms, primarily neurofibromas, schwannomas and maliganant schwannomas, are among the most common tumors in fishes. Model systems involving PNS tumors in fishes are also valuable because mammalian models of PNS tumors are rare. Schwann cells, the primary cell type suspected of neoplastic transformation in these tumors, have been difficult to culture. We describe techniques for culturing normal and neoplastic Schwann cells from fish. We also present methods for preparing cells on culture dishes for electron microscopy which are especially useful when specific cells in a culture must be located for ultrastructural examination.

Original languageEnglish (US)
Pages (from-to)109-115
Number of pages7
JournalJournal of Tissue Culture Methods
Volume16
Issue number2
DOIs
StatePublished - Jun 1 1994

Keywords

  • Animal model
  • Neurofibroma
  • Schwann cell
  • Ultrastructure

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Fingerprint Dive into the research topics of 'Normal and neoplastic Schwann cells in fish - Isolation, culture and preparation for electron microscopy'. Together they form a unique fingerprint.

Cite this