Normal and neoplastic Schwann cells in fish - Isolation, culture and preparation for electron microscopy

Michael C Schmale, Kenneth A. Gill, Saul M. Cacal

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Peripheral nerve sheath (PNS) neoplasms, primarily neurofibromas, schwannomas and maliganant schwannomas, are among the most common tumors in fishes. Model systems involving PNS tumors in fishes are also valuable because mammalian models of PNS tumors are rare. Schwann cells, the primary cell type suspected of neoplastic transformation in these tumors, have been difficult to culture. We describe techniques for culturing normal and neoplastic Schwann cells from fish. We also present methods for preparing cells on culture dishes for electron microscopy which are especially useful when specific cells in a culture must be located for ultrastructural examination.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalJournal of Tissue Culture Methods
Volume16
Issue number2
DOIs
StatePublished - Jun 1 1994

Fingerprint

Nerve Sheath Neoplasms
Schwann Cells
Cell culture
Fish
Electron microscopy
Tumors
Electron Microscopy
Fishes
Neurilemmoma
Cells
Peripheral Nervous System Neoplasms
Neoplastic Cell Transformation
Neurofibroma
Neoplasms
Cell Culture Techniques

Keywords

  • Animal model
  • Neurofibroma
  • Schwann cell
  • Ultrastructure

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Cite this

Normal and neoplastic Schwann cells in fish - Isolation, culture and preparation for electron microscopy. / Schmale, Michael C; Gill, Kenneth A.; Cacal, Saul M.

In: Journal of Tissue Culture Methods, Vol. 16, No. 2, 01.06.1994, p. 109-115.

Research output: Contribution to journalArticle

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