Abstract
Norepinephrine stimulated the rapid hydrolysis of [3H]phosphatidylinositol-4,5-bisphosphate in rat aorta with a maximal decrease of 30% within 60 sec of stimulation. Levels of [3H]phosphatidylinositol-4,5-bisphosphate returned to control by 5 min despite the continued presence of agonist. Hydrolysis of [3H]phosphatidylinositol-4,5-bisphosphate occurred concurrently with the formation of inositol phosphates. Inositol-tris and tetrakisphosphate levels were increased within 30 sec of agonist stimulation. Increases in inositol phosphate levels due to agonist were dose-dependent with half-maximal activation at 1 μM norepinephrine.
Original language | English (US) |
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Pages (from-to) | 240-245 |
Number of pages | 6 |
Journal | Biochemical and biophysical research communications |
Volume | 156 |
Issue number | 1 |
DOIs | |
State | Published - Oct 14 1988 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology