Nontoxic carbon dots potently inhibit human insulin fibrillation

Shanghao Li, Lingyu Wang, Charles C. Chusuei, Valentina M. Suarez, Patrica L. Blackwelder, Miodrag Micic, Jhony Orbulescu, Roger Leblanc

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

One prevention and therapeutic strategy for diseases associated with peptide or protein fibrillation is to inhibit or delay the fibrillation process. Carbon dots (C-Dots) have recently emerged as benign nanoparticles to replace toxic quantum dots and have attracted great attention because of their unique optical properties and potential applications in biological systems. However, the effect of C-Dots on peptide or protein fibrillation has not been explored. In this in vitro study, human insulin was selected as a model to investigate the effect of C-Dots on insulin fibrillation. Water-soluble fluorescent C-Dots with sizes less than 6 nm were prepared from carbon powder and characterized by UV-vis spectroscopy, fluorescence, Fourier transform infrared spectrophotometry, X-ray photoelectron spectrometry, transmission electron microscopy, and atomic force microscopy. These C-Dots were able to efficiently inhibit insulin fibrillation in a concentration-dependent manner. The inhibiting effect of C-Dots was even observed at 0.2 μg/mL. Importantly, 40 μg/mL of C-Dots prevent 0.2 mg/mL of human insulin from fibrillation for 5 days under 65 °C, whereas insulin denatures in 3 h under the same conditions without C-Dots. The inhibiting effect is likely due to the interaction between C-Dots and insulin species before elongation. Cytotoxicity study shows that these C-Dots have very low cytotoxicity. Therefore, these C-Dots have the potential to inhibit insulin fibrillation in biological systems and in the pharmaceutical industry for the processing and formulation of insulin.

Original languageEnglish (US)
Pages (from-to)1764-1771
Number of pages8
JournalChemistry of Materials
Volume27
Issue number5
DOIs
StatePublished - Mar 10 2015

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Insulin
Carbon
Biological systems
Cytotoxicity
Peptides
Proteins
Poisons
Spectrophotometry
Photoelectrons
Ultraviolet spectroscopy
Powders
Drug products
Spectrometry
Semiconductor quantum dots
Elongation
Atomic force microscopy
Fourier transforms
Optical properties
Fluorescence
Nanoparticles

ASJC Scopus subject areas

  • Materials Chemistry
  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Li, S., Wang, L., Chusuei, C. C., Suarez, V. M., Blackwelder, P. L., Micic, M., ... Leblanc, R. (2015). Nontoxic carbon dots potently inhibit human insulin fibrillation. Chemistry of Materials, 27(5), 1764-1771. https://doi.org/10.1021/cm504572b

Nontoxic carbon dots potently inhibit human insulin fibrillation. / Li, Shanghao; Wang, Lingyu; Chusuei, Charles C.; Suarez, Valentina M.; Blackwelder, Patrica L.; Micic, Miodrag; Orbulescu, Jhony; Leblanc, Roger.

In: Chemistry of Materials, Vol. 27, No. 5, 10.03.2015, p. 1764-1771.

Research output: Contribution to journalArticle

Li, S, Wang, L, Chusuei, CC, Suarez, VM, Blackwelder, PL, Micic, M, Orbulescu, J & Leblanc, R 2015, 'Nontoxic carbon dots potently inhibit human insulin fibrillation', Chemistry of Materials, vol. 27, no. 5, pp. 1764-1771. https://doi.org/10.1021/cm504572b
Li S, Wang L, Chusuei CC, Suarez VM, Blackwelder PL, Micic M et al. Nontoxic carbon dots potently inhibit human insulin fibrillation. Chemistry of Materials. 2015 Mar 10;27(5):1764-1771. https://doi.org/10.1021/cm504572b
Li, Shanghao ; Wang, Lingyu ; Chusuei, Charles C. ; Suarez, Valentina M. ; Blackwelder, Patrica L. ; Micic, Miodrag ; Orbulescu, Jhony ; Leblanc, Roger. / Nontoxic carbon dots potently inhibit human insulin fibrillation. In: Chemistry of Materials. 2015 ; Vol. 27, No. 5. pp. 1764-1771.
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