Nonossifying fibroma of long bones. An immunohistochemical study

P. A. Bejarano, M. Kyriakos

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Fourteen cases of nonossifying fibroma (NOF) of bone were studied immunohistochemically to elucidate the character of their constituent stromal cells, multinucleated osteoclastic-type giant cells, and foam cells. The stromal spindle cells, the main cellular component of NOF, stained for KP-1 (CD-68), α-1-antichymotrypsin, and HAM-56 in ail cases and were immunoreactive for α-1-antitrypsin and HLA-DR in 13 (93%) cases and for lysozyme in 8 cases (57%). These results indicate that the cells have a histiocytic immunophenotype. However, they also expressed vimentin and muscle-specific actin (MSA) in 14 (100%) and 13 (93%) cases, respectively, indicating that they might also have fibroblastic or myofibroblastic characteristics. This histiocytic-mesenchymal immunophenotype was present in most of the stromal cells; from 50 to 100% of them coexpressed KP-1, vimentin, and MSA. The stromal cells were essentially the only cells reactive for proliferating cell nuclear antigen (PCNA) and Ki-67 antigen, which were present in the cells of 13 (93%) and 10 (77%) of the cases, respectively. The multinucleated giant cells and foam cells also showed immunohistochemical evidence of histiocytic differentiation. The former were reactive for KP-1 in all 14 cases, for lysozyme in 13 (93%), and for α-1-antichymotrypsin in 8 of 13 cases (62%). Foam cells expressed reactivity for KP-1, lysozyme, HLA-DR, and HAM-56 in all of the cases in which they were present, whereas leukocyte common antigen (LCA) expression was present in 66% of the cases. The findings in our study indicate that the stromal cells in nonossifying fibroma are the proliferative elements and that they possess immunohistochemical feature of both histiocytic and fribroblastic-myofibroblastic differentiation. The foam and multinucleated giant cells appear to represent an end stage of cellular development lacking proliferative properties; their origins appear linked to that of the stromal cells. There was no support for the suggestion that NOF is a lipoblastic lesion, as all cells were nonreactive for S-100 protein.

Original languageEnglish
Pages (from-to)257-264
Number of pages8
JournalApplied Immunohistochemistry
Volume3
Issue number4
StatePublished - Jan 1 1995

Fingerprint

Fibroma
Stromal Cells
Bone and Bones
Foam Cells
Giant Cells
Muramidase
HLA-DR Antigens
Vimentin
Actins
Ki-67 Antigen
CD45 Antigens
Muscles
S100 Proteins
Proliferating Cell Nuclear Antigen

Keywords

  • Fibroblast
  • Histiocyte
  • Immunohistochemistry
  • Metaphyseal fibrous defect
  • Myofibroblast
  • Nonossifying fibroma

ASJC Scopus subject areas

  • Anatomy
  • Medical Laboratory Technology

Cite this

Nonossifying fibroma of long bones. An immunohistochemical study. / Bejarano, P. A.; Kyriakos, M.

In: Applied Immunohistochemistry, Vol. 3, No. 4, 01.01.1995, p. 257-264.

Research output: Contribution to journalArticle

Bejarano, PA & Kyriakos, M 1995, 'Nonossifying fibroma of long bones. An immunohistochemical study', Applied Immunohistochemistry, vol. 3, no. 4, pp. 257-264.
Bejarano, P. A. ; Kyriakos, M. / Nonossifying fibroma of long bones. An immunohistochemical study. In: Applied Immunohistochemistry. 1995 ; Vol. 3, No. 4. pp. 257-264.
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