Noninvasive in vivo imaging of pancreatic islet cell biology

Stephan Speier, Daniel Nyqvist, Over Cabrera, Jia Yu, R. Damaris Molano, Antonello Pileggi, Tilo Moede, Martin Köhler, Johannes Wilbertz, Barbara Leibiger, Camillo Ricordi, Ingo B. Leibiger, Alejandro Caicedo, Per Olof Berggren

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161 Scopus citations

Abstract

Advanced imaging techniques have become a valuable tool in the study of complex biological processes at the cellular level in biomedical research. Here, we introduce a new technical platform for noninvasive in vivo fluorescence imaging of pancreatic islets using the anterior chamber of the eye as a natural body window. Islets transplanted into the mouse eye engrafted on the iris, became vascularized, retained cellular composition, responded to stimulation and reverted diabetes. Laser-scanning microscopy allowed repetitive in vivo imaging of islet vascularization, beta cell function and death at cellular resolution. Our results thus establish the basis for noninvasive in vivo investigations of complex cellular processes, like beta cell stimulus-response coupling, which can be performed longitudinally under both physiological and pathological conditions.

Original languageEnglish (US)
Pages (from-to)574-578
Number of pages5
JournalNature medicine
Volume14
Issue number5
DOIs
StatePublished - May 1 2008

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Speier, S., Nyqvist, D., Cabrera, O., Yu, J., Molano, R. D., Pileggi, A., Moede, T., Köhler, M., Wilbertz, J., Leibiger, B., Ricordi, C., Leibiger, I. B., Caicedo, A., & Berggren, P. O. (2008). Noninvasive in vivo imaging of pancreatic islet cell biology. Nature medicine, 14(5), 574-578. https://doi.org/10.1038/nm1701