Abstract
Background: Although evidence has shown that very small electrical currents produce a beneficial therapeutic result for wounds, noninvasive electromagnetic field (EMF) therapy has consisted mostly of anecdotal clinical reports, with very few well-controlled laboratory mechanistic studies. In this study, we evaluate the effects and potential mechanisms of a noninvasive EMF device on skin wound repair. Materials and Methods: The effects of noninvasive EMF on keratinocytes and fibroblasts were assessed via proliferation and incisional wound model migration assays. cDNA microarray and RT-PCR were utilized to assess genetic expression changes in keratinocytes after noninvasive EMF treatment. Results: In vitro analyses with human skin kerati-nocyte cultures demonstrated that noninvasive EMFs have a strong effect on accelerating keratino-cyte migration and a relatively weaker effect on promoting keratinocyte proliferation. The positive effects of noninvasive EMFs on cell migration and proliferation seem keratinocyte-specific without such effects seen on dermal fibroblasts. cDNA micro-array and RT-PCR performed revealed increased expression of CRK7 and HOXC8 genes in treated keratinocytes. Conclusions: This study suggests that a noninvasive EMF accelerates wound re-epithelialization through a mechanism of promoting keratinocyte migration and proliferation, possibly due to upregulation of CRK7 and HOXC8 genes.
| Original language | English |
|---|---|
| Pages (from-to) | 299-307 |
| Number of pages | 9 |
| Journal | Journal of Surgical Research |
| Volume | 162 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 1 2010 |
Fingerprint
Keywords
- CDNA
- Electromagnetic field
- Fibroblast
- Keratinocyte
- Migration
- Proliferation
- RT-PCR
- Wound healing
ASJC Scopus subject areas
- Surgery
Cite this
Noninvasive electromagnetic fields on keratinocyte growth and migration. / Huo, Ran; Ma, Qianli; Wu, James J.; Chin-Nuke, Kayla; Jing, Yuqi; Chen, Juan; Miyar, Maria E.; Davis, Stephen C; Li, Jie.
In: Journal of Surgical Research, Vol. 162, No. 2, 01.08.2010, p. 299-307.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Noninvasive electromagnetic fields on keratinocyte growth and migration
AU - Huo, Ran
AU - Ma, Qianli
AU - Wu, James J.
AU - Chin-Nuke, Kayla
AU - Jing, Yuqi
AU - Chen, Juan
AU - Miyar, Maria E.
AU - Davis, Stephen C
AU - Li, Jie
PY - 2010/8/1
Y1 - 2010/8/1
N2 - Background: Although evidence has shown that very small electrical currents produce a beneficial therapeutic result for wounds, noninvasive electromagnetic field (EMF) therapy has consisted mostly of anecdotal clinical reports, with very few well-controlled laboratory mechanistic studies. In this study, we evaluate the effects and potential mechanisms of a noninvasive EMF device on skin wound repair. Materials and Methods: The effects of noninvasive EMF on keratinocytes and fibroblasts were assessed via proliferation and incisional wound model migration assays. cDNA microarray and RT-PCR were utilized to assess genetic expression changes in keratinocytes after noninvasive EMF treatment. Results: In vitro analyses with human skin kerati-nocyte cultures demonstrated that noninvasive EMFs have a strong effect on accelerating keratino-cyte migration and a relatively weaker effect on promoting keratinocyte proliferation. The positive effects of noninvasive EMFs on cell migration and proliferation seem keratinocyte-specific without such effects seen on dermal fibroblasts. cDNA micro-array and RT-PCR performed revealed increased expression of CRK7 and HOXC8 genes in treated keratinocytes. Conclusions: This study suggests that a noninvasive EMF accelerates wound re-epithelialization through a mechanism of promoting keratinocyte migration and proliferation, possibly due to upregulation of CRK7 and HOXC8 genes.
AB - Background: Although evidence has shown that very small electrical currents produce a beneficial therapeutic result for wounds, noninvasive electromagnetic field (EMF) therapy has consisted mostly of anecdotal clinical reports, with very few well-controlled laboratory mechanistic studies. In this study, we evaluate the effects and potential mechanisms of a noninvasive EMF device on skin wound repair. Materials and Methods: The effects of noninvasive EMF on keratinocytes and fibroblasts were assessed via proliferation and incisional wound model migration assays. cDNA microarray and RT-PCR were utilized to assess genetic expression changes in keratinocytes after noninvasive EMF treatment. Results: In vitro analyses with human skin kerati-nocyte cultures demonstrated that noninvasive EMFs have a strong effect on accelerating keratino-cyte migration and a relatively weaker effect on promoting keratinocyte proliferation. The positive effects of noninvasive EMFs on cell migration and proliferation seem keratinocyte-specific without such effects seen on dermal fibroblasts. cDNA micro-array and RT-PCR performed revealed increased expression of CRK7 and HOXC8 genes in treated keratinocytes. Conclusions: This study suggests that a noninvasive EMF accelerates wound re-epithelialization through a mechanism of promoting keratinocyte migration and proliferation, possibly due to upregulation of CRK7 and HOXC8 genes.
KW - CDNA
KW - Electromagnetic field
KW - Fibroblast
KW - Keratinocyte
KW - Migration
KW - Proliferation
KW - RT-PCR
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=77955983629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955983629&partnerID=8YFLogxK
U2 - 10.1016/j.jss.2009.02.016
DO - 10.1016/j.jss.2009.02.016
M3 - Article
C2 - 19592020
AN - SCOPUS:77955983629
VL - 162
SP - 299
EP - 307
JO - Journal of Surgical Research
JF - Journal of Surgical Research
SN - 0022-4804
IS - 2
ER -