Noninvasive electromagnetic fields on keratinocyte growth and migration

Ran Huo, Qianli Ma, James J. Wu, Kayla Chin-Nuke, Yuqi Jing, Juan Chen, Maria E. Miyar, Stephen C Davis, Jie Li

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: Although evidence has shown that very small electrical currents produce a beneficial therapeutic result for wounds, noninvasive electromagnetic field (EMF) therapy has consisted mostly of anecdotal clinical reports, with very few well-controlled laboratory mechanistic studies. In this study, we evaluate the effects and potential mechanisms of a noninvasive EMF device on skin wound repair. Materials and Methods: The effects of noninvasive EMF on keratinocytes and fibroblasts were assessed via proliferation and incisional wound model migration assays. cDNA microarray and RT-PCR were utilized to assess genetic expression changes in keratinocytes after noninvasive EMF treatment. Results: In vitro analyses with human skin kerati-nocyte cultures demonstrated that noninvasive EMFs have a strong effect on accelerating keratino-cyte migration and a relatively weaker effect on promoting keratinocyte proliferation. The positive effects of noninvasive EMFs on cell migration and proliferation seem keratinocyte-specific without such effects seen on dermal fibroblasts. cDNA micro-array and RT-PCR performed revealed increased expression of CRK7 and HOXC8 genes in treated keratinocytes. Conclusions: This study suggests that a noninvasive EMF accelerates wound re-epithelialization through a mechanism of promoting keratinocyte migration and proliferation, possibly due to upregulation of CRK7 and HOXC8 genes.

Original languageEnglish
Pages (from-to)299-307
Number of pages9
JournalJournal of Surgical Research
Volume162
Issue number2
DOIs
StatePublished - Aug 1 2010

Fingerprint

Electromagnetic Fields
Keratinocytes
Growth
Re-Epithelialization
Oligonucleotide Array Sequence Analysis
Skin
Wounds and Injuries
Fibroblasts
Polymerase Chain Reaction
Genes
Cell Movement
Up-Regulation
Cell Proliferation
Equipment and Supplies
Therapeutics

Keywords

  • CDNA
  • Electromagnetic field
  • Fibroblast
  • Keratinocyte
  • Migration
  • Proliferation
  • RT-PCR
  • Wound healing

ASJC Scopus subject areas

  • Surgery

Cite this

Noninvasive electromagnetic fields on keratinocyte growth and migration. / Huo, Ran; Ma, Qianli; Wu, James J.; Chin-Nuke, Kayla; Jing, Yuqi; Chen, Juan; Miyar, Maria E.; Davis, Stephen C; Li, Jie.

In: Journal of Surgical Research, Vol. 162, No. 2, 01.08.2010, p. 299-307.

Research output: Contribution to journalArticle

Huo, R, Ma, Q, Wu, JJ, Chin-Nuke, K, Jing, Y, Chen, J, Miyar, ME, Davis, SC & Li, J 2010, 'Noninvasive electromagnetic fields on keratinocyte growth and migration', Journal of Surgical Research, vol. 162, no. 2, pp. 299-307. https://doi.org/10.1016/j.jss.2009.02.016
Huo, Ran ; Ma, Qianli ; Wu, James J. ; Chin-Nuke, Kayla ; Jing, Yuqi ; Chen, Juan ; Miyar, Maria E. ; Davis, Stephen C ; Li, Jie. / Noninvasive electromagnetic fields on keratinocyte growth and migration. In: Journal of Surgical Research. 2010 ; Vol. 162, No. 2. pp. 299-307.
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