Noninvasive assessments of optic nerve neurodegeneration in transgenic mice with isolated optic neuritis

Venu Talla, Cui Yang, Gerry Shaw, Vittorio Porciatti, Rajeshwari D. Koilkonda, John Guy

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


PURPOSE. To determine if phosphorylated neurofilament heavy chain (pNF-H) released into the bloodstream and the pattern ERG are noninvasive indicators of neurodegeneration in experimental optic neuritis. METHODS. Serum from Myelin oligodendrocyte glycoprotein (MOG)-specific T cell receptor-positive (TCR{thorn}) transgenic mice that develop isolated optic neuritis usually without any other characteristic lesions of inflammation or demyelination in the spinal cord and littermates negative for the transgene were assayed for the presence of serum phosphorylated neurofilament H (pNF-H). In vivo measurements of optic nerve and retinal ganglion cell injury were assessed by magnetic resonance imaging (MRI), optical coherence tomography (OCT), and pattern electroretinogram (PERG). Automated two dimensional fluorescence differential in-gel electrophoresis (2D-DIGE) of pooled optic nerve samples, light, and transmission electron micrographs were used to evaluate optic atrophy postmortem. RESULTS. We found an almost 3-fold elevation in serum pNF-H levels in MOG+ mice relative to MOG-littermates (P = 0.02). 2D-DIGE revealed a 3-fold reduction in optic nerve neurofilaments. Visual function assessed by the PERG was reduced by one-quarter (P = 0.033) and latencies increased by 38% (P = 0.036). MOG+ mice with the lowest PERG amplitudes had optic nerve atrophy visualized by MRI. Optic nerve diameters were reduced by one-third (P = 0.0001) and axon counts reduced by more than two-thirds. Histopathology of the spinal cords was normal. CONCLUSIONS. Elevated serum pNF-H levels and the PERG are useful markers of neurodegeneration of the optic nerve in isolated experimental optic neuritis. Our findings suggest that elevations of this axonal protein in patients with optic neuritis who had a poor visual outcome are likely also due to demise of optic nerve axons.

Original languageEnglish (US)
Pages (from-to)4440-4450
Number of pages11
JournalInvestigative Ophthalmology and Visual Science
Issue number7
StatePublished - 2013


  • Axonal degeneration
  • Neurodegeneration
  • Neurofilaments
  • Neuroophthalmology
  • Optic neuritis

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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