Noninfectious sporozoites in the salivary glands of a minimally susceptible anopheline mosquito

B. H. Noden, C. B. Pumpuni, J. A. Vaughan, John C Beier

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In studies to evaluate vector-malaria parasite relationships, we have found that Anopheles albimanus is minimally susceptible to the rodent malaria parasite Plasmodium yoelii. Normally, less than 10% of A. albimanus develop oocyst infections compared to 80-100% for Anopheles stephensi and Anopheles freeborni mosquitoes. Although sporozoites produced in A. albimanus invade the salivary glands, they are not infectious to BALB/c or ICR mice. In 11 experiments with sporozoites from A. albimanus, intravenous inoculations of up to 24,000 sporozoites in individual mice failed to produce host infections. In contrast, inoculation of 300 sporozoites obtained from the salivary glands of A. stephensi and A. freeborni always infected mice. The noninfectious sporozoites from A. albimanus were morphologically similar to the infectious sporozoites from A. stephensi and yielded 4+ circumsporozoite precipitin reactions when incubated with a monoclonal antibody against the circumsporozoite protein of P. yoelii. The presence of noninfectious sporozoites in the salivary glands of A. albimanus suggests that this minimally susceptible vector either possesses a toxic factor that abolishes sporozoite infectiousness or lacks a critical substance needed by the sporozoite to become infectious. Sporozoite infectiousness was neither attenuated by incubation of infectious sporozoites with A. albimanus salivary glands nor restored when noninfectious sporozoites were incubated with A. stephensi salivary glands. These studies provide a starling point for defining the biological basis of sporozoite infectivity.

Original languageEnglish
Pages (from-to)912-915
Number of pages4
JournalJournal of Parasitology
Volume81
Issue number6
DOIs
StatePublished - Dec 1 1995
Externally publishedYes

Fingerprint

Sporozoites
sporozoites
malaria
salivary glands
Salivary Glands
Culicidae
mosquito
inoculation
parasite
Anopheles albimanus
infectivity
rodent
antibody
Anopheles stephensi
incubation
protein
Anopheles
Anopheles freeborni
Plasmodium yoelii
experiment

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Noninfectious sporozoites in the salivary glands of a minimally susceptible anopheline mosquito. / Noden, B. H.; Pumpuni, C. B.; Vaughan, J. A.; Beier, John C.

In: Journal of Parasitology, Vol. 81, No. 6, 01.12.1995, p. 912-915.

Research output: Contribution to journalArticle

Noden, B. H. ; Pumpuni, C. B. ; Vaughan, J. A. ; Beier, John C. / Noninfectious sporozoites in the salivary glands of a minimally susceptible anopheline mosquito. In: Journal of Parasitology. 1995 ; Vol. 81, No. 6. pp. 912-915.
@article{a45a8ddf7ad94ea798bdca1dcdc3d77c,
title = "Noninfectious sporozoites in the salivary glands of a minimally susceptible anopheline mosquito",
abstract = "In studies to evaluate vector-malaria parasite relationships, we have found that Anopheles albimanus is minimally susceptible to the rodent malaria parasite Plasmodium yoelii. Normally, less than 10{\%} of A. albimanus develop oocyst infections compared to 80-100{\%} for Anopheles stephensi and Anopheles freeborni mosquitoes. Although sporozoites produced in A. albimanus invade the salivary glands, they are not infectious to BALB/c or ICR mice. In 11 experiments with sporozoites from A. albimanus, intravenous inoculations of up to 24,000 sporozoites in individual mice failed to produce host infections. In contrast, inoculation of 300 sporozoites obtained from the salivary glands of A. stephensi and A. freeborni always infected mice. The noninfectious sporozoites from A. albimanus were morphologically similar to the infectious sporozoites from A. stephensi and yielded 4+ circumsporozoite precipitin reactions when incubated with a monoclonal antibody against the circumsporozoite protein of P. yoelii. The presence of noninfectious sporozoites in the salivary glands of A. albimanus suggests that this minimally susceptible vector either possesses a toxic factor that abolishes sporozoite infectiousness or lacks a critical substance needed by the sporozoite to become infectious. Sporozoite infectiousness was neither attenuated by incubation of infectious sporozoites with A. albimanus salivary glands nor restored when noninfectious sporozoites were incubated with A. stephensi salivary glands. These studies provide a starling point for defining the biological basis of sporozoite infectivity.",
author = "Noden, {B. H.} and Pumpuni, {C. B.} and Vaughan, {J. A.} and Beier, {John C}",
year = "1995",
month = "12",
day = "1",
doi = "10.2307/3284039",
language = "English",
volume = "81",
pages = "912--915",
journal = "Journal of Parasitology",
issn = "0022-3395",
publisher = "American Society of Parasitologists",
number = "6",

}

TY - JOUR

T1 - Noninfectious sporozoites in the salivary glands of a minimally susceptible anopheline mosquito

AU - Noden, B. H.

AU - Pumpuni, C. B.

AU - Vaughan, J. A.

AU - Beier, John C

PY - 1995/12/1

Y1 - 1995/12/1

N2 - In studies to evaluate vector-malaria parasite relationships, we have found that Anopheles albimanus is minimally susceptible to the rodent malaria parasite Plasmodium yoelii. Normally, less than 10% of A. albimanus develop oocyst infections compared to 80-100% for Anopheles stephensi and Anopheles freeborni mosquitoes. Although sporozoites produced in A. albimanus invade the salivary glands, they are not infectious to BALB/c or ICR mice. In 11 experiments with sporozoites from A. albimanus, intravenous inoculations of up to 24,000 sporozoites in individual mice failed to produce host infections. In contrast, inoculation of 300 sporozoites obtained from the salivary glands of A. stephensi and A. freeborni always infected mice. The noninfectious sporozoites from A. albimanus were morphologically similar to the infectious sporozoites from A. stephensi and yielded 4+ circumsporozoite precipitin reactions when incubated with a monoclonal antibody against the circumsporozoite protein of P. yoelii. The presence of noninfectious sporozoites in the salivary glands of A. albimanus suggests that this minimally susceptible vector either possesses a toxic factor that abolishes sporozoite infectiousness or lacks a critical substance needed by the sporozoite to become infectious. Sporozoite infectiousness was neither attenuated by incubation of infectious sporozoites with A. albimanus salivary glands nor restored when noninfectious sporozoites were incubated with A. stephensi salivary glands. These studies provide a starling point for defining the biological basis of sporozoite infectivity.

AB - In studies to evaluate vector-malaria parasite relationships, we have found that Anopheles albimanus is minimally susceptible to the rodent malaria parasite Plasmodium yoelii. Normally, less than 10% of A. albimanus develop oocyst infections compared to 80-100% for Anopheles stephensi and Anopheles freeborni mosquitoes. Although sporozoites produced in A. albimanus invade the salivary glands, they are not infectious to BALB/c or ICR mice. In 11 experiments with sporozoites from A. albimanus, intravenous inoculations of up to 24,000 sporozoites in individual mice failed to produce host infections. In contrast, inoculation of 300 sporozoites obtained from the salivary glands of A. stephensi and A. freeborni always infected mice. The noninfectious sporozoites from A. albimanus were morphologically similar to the infectious sporozoites from A. stephensi and yielded 4+ circumsporozoite precipitin reactions when incubated with a monoclonal antibody against the circumsporozoite protein of P. yoelii. The presence of noninfectious sporozoites in the salivary glands of A. albimanus suggests that this minimally susceptible vector either possesses a toxic factor that abolishes sporozoite infectiousness or lacks a critical substance needed by the sporozoite to become infectious. Sporozoite infectiousness was neither attenuated by incubation of infectious sporozoites with A. albimanus salivary glands nor restored when noninfectious sporozoites were incubated with A. stephensi salivary glands. These studies provide a starling point for defining the biological basis of sporozoite infectivity.

UR - http://www.scopus.com/inward/record.url?scp=0029564672&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029564672&partnerID=8YFLogxK

U2 - 10.2307/3284039

DO - 10.2307/3284039

M3 - Article

C2 - 8544063

AN - SCOPUS:0029564672

VL - 81

SP - 912

EP - 915

JO - Journal of Parasitology

JF - Journal of Parasitology

SN - 0022-3395

IS - 6

ER -