Non-Killer Cell-Specific Transcription Factors Silence the Perforin Promoter

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23 Scopus citations

Abstract

Perforin is a pore-forming killer protein exclusively expressed by cells functionally defined as cytolytic lymphocytes. Previous reporter gene investigations have indicated that the cell-type-specific expression of the perform gene is determined by the promoter and upstream region but the respective cis- and trans-acting molecules remain to be identified. In this investigation, we differentially display DNA-protein interactions of perforin-positive vs perforin-negative cell types and functionally test their biological relevance. Our results provide molecular evidence for the transcriptional repression of the perform gene in non-killer cells by two novel regulatory elements. One of the respective transcription factors that are exclusively expressed by non-killer cells appears to be an Ets family member. Thus, the development of cells expressing perforin, and perhaps cytolytic lymphocytes in general, may involve a shutdown of the genes for these proteins.

Original languageEnglish (US)
Pages (from-to)1734-1741
Number of pages8
JournalJournal of Immunology
Volume158
Issue number4
StatePublished - Feb 15 1997

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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