Non-invasive longitudinal tracking of human amniotic fluid stem cells in the mouse heart

Dawn M. Delo, John Olson, Pedro M. Baptista, Ralph B. D'Agostino, Anthony Atala, Jian Ming Zhu, Shay Soker

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Human stem cells from various sources have potential therapeutic applications. The clinical implementation of these therapies introduces the need for methods of noninvasive tracking of cells. The purpose of this study was to evaluate a high resolution magnetic resonance imaging (MRI) technique for in vivo detection and tracking of superparamagnetic micron sized iron oxide particle (MPIO)-labeled human amniotic fluid stem (hAFS) cells injected in the mouse heart. Because of the small subject size, MR signal and resolution of the in vivo MRI were increased using strong gradients, a 7.0 Tesla magnet, and an ECG and respiratory gated gradient echo sequence. MRI images of mouse heart were acquired during a 4 week course of this longitudinal study. At the end of the study, histological analysis was used to correlate cell localization with the MRI results. Introduction of MPIOs into hAFS had no significant effect upon cell proliferation and differentiation. Results of flow cytometry analysis indicated that hAFS cells remained labeled for up to 4 weeks. MRI of MPIO-labeled hAFS cells injected in agarose gels resulted in significant hypointense regions. Labeled hAFS cells injected into mouse hearts produced hypointense regions in the MR images that could be detected 24 hours and 7, 14, 21 and 28 days post injection. The co-localization of labeled cells within the hypointense regions was confirmed by histological analysis. These results indicate that high resolution MRI can be used successfully for noninvasive longitudinal tracking of hAFS cells injected in the mouse heart. The potential utility of this finding is that injected stem cells can be tracked in vivo and might serve to monitor cell survival, proliferation and integration into myocardial tissue.

Original languageEnglish
Pages (from-to)1185-1193
Number of pages9
JournalStem Cells and Development
Volume17
Issue number6
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

Fingerprint

Amniotic Fluid
Stem Cells
Magnetic Resonance Imaging
Cell Proliferation
Cell Tracking
Magnets
Sepharose
Longitudinal Studies
Cell Differentiation
Cell Survival
Flow Cytometry
Electrocardiography
Gels
Injections
Therapeutics

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

Cite this

Delo, D. M., Olson, J., Baptista, P. M., D'Agostino, R. B., Atala, A., Zhu, J. M., & Soker, S. (2008). Non-invasive longitudinal tracking of human amniotic fluid stem cells in the mouse heart. Stem Cells and Development, 17(6), 1185-1193. https://doi.org/10.1089/scd.2008.0028

Non-invasive longitudinal tracking of human amniotic fluid stem cells in the mouse heart. / Delo, Dawn M.; Olson, John; Baptista, Pedro M.; D'Agostino, Ralph B.; Atala, Anthony; Zhu, Jian Ming; Soker, Shay.

In: Stem Cells and Development, Vol. 17, No. 6, 01.12.2008, p. 1185-1193.

Research output: Contribution to journalArticle

Delo, DM, Olson, J, Baptista, PM, D'Agostino, RB, Atala, A, Zhu, JM & Soker, S 2008, 'Non-invasive longitudinal tracking of human amniotic fluid stem cells in the mouse heart', Stem Cells and Development, vol. 17, no. 6, pp. 1185-1193. https://doi.org/10.1089/scd.2008.0028
Delo DM, Olson J, Baptista PM, D'Agostino RB, Atala A, Zhu JM et al. Non-invasive longitudinal tracking of human amniotic fluid stem cells in the mouse heart. Stem Cells and Development. 2008 Dec 1;17(6):1185-1193. https://doi.org/10.1089/scd.2008.0028
Delo, Dawn M. ; Olson, John ; Baptista, Pedro M. ; D'Agostino, Ralph B. ; Atala, Anthony ; Zhu, Jian Ming ; Soker, Shay. / Non-invasive longitudinal tracking of human amniotic fluid stem cells in the mouse heart. In: Stem Cells and Development. 2008 ; Vol. 17, No. 6. pp. 1185-1193.
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