Non-contact optical measurement of lens capsule thickness during simulated accommodation

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Purpose: To non-invasively measure the thickness of the anterior and posterior lens capsule, and to determine if it significantly changes during accommodation. Methods: Anterior and posterior capsule thickness was measured on post-mortem lenses using a non-contact optical system using a focus-detection technique. The optical system uses a 670nm laser beam delivered to a single-mode fiber coupler. The output of the fiber coupler is focused on the tissue surface using an aspheric lens (NA=0.68) mounted on a translation stage with a motorized actuator. Light reflected from the sample surface is collected by the fiber coupler and sent to a photoreceiver connected to a computer-controlled data acquisition system. Optical intensity peaks are detected when the aspheric lens is focused on the capsule boundaries. The capsule thickness is equal to the distance traveled between two peaks multiplied by the capsule refractive index. Anterior and posterior lens capsule thickness measurements were performed on 18 cynomolgus (age average: 6±1 years, range: 47 years) eyes, 1 rhesus (age: 2 years) eye, and 12 human (age average: 65±16, range: 47-92) eyes during simulated accommodation. The mounted sample was placed under the focusing objective of the optical system so that the light was incident on the center pole. Measurements were taken of the anterior lens capsule in the unstretched and the stretched 5mm states. The lens was flipped, and the same procedure was performed for the posterior lens capsule. Results: The precision of the optical system was determined to be ±0.5μm. The resolution is 4μm and the sensitivity is 52dB. The human anterior lens capsule thickness was 6.0±1.2μm unstretched and 4.9±0.9μm stretched (p=0.008). The human posterior lens capsule was 5.7±1.2μm unstretched and 5.7±1.4μm stretched (p=0.974). The monkey anterior lens capsule thickness was 5.9±1.9μm unstretched and 4.8±1.0μm stretched (p=0.002). The monkey posterior lens capsule was 5.9±2.0μm unstretched and 5.1±1.3μm stretched (p=0.128). Conclusions: The results indicate that the primate anterior lens capsule thickness changes during accommodation.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsF. Manns, P.G. Soederberg, A. Ho, B.E. Stuck, M. Belkin
Pages19-25
Number of pages7
Volume5688
DOIs
StatePublished - 2005
EventOphtalmic Technologies XV - San Jose, CA, United States
Duration: Jan 22 2005Jan 25 2005

Other

OtherOphtalmic Technologies XV
CountryUnited States
CitySan Jose, CA
Period1/22/051/25/05

Fingerprint

Lenses
Optical systems
Thickness measurement
Fibers
Single mode fibers
Laser beams
Poles
Data acquisition
Refractive index
Actuators
Tissue

Keywords

  • Accommodation
  • Lens capsule
  • Presbyopia

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Ziebarth, N. M., Manns, F., Acosta, A. C., & Parel, J-M. A. (2005). Non-contact optical measurement of lens capsule thickness during simulated accommodation. In F. Manns, P. G. Soederberg, A. Ho, B. E. Stuck, & M. Belkin (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 5688, pp. 19-25). [05] https://doi.org/10.1117/12.597648

Non-contact optical measurement of lens capsule thickness during simulated accommodation. / Ziebarth, Noel Marysa; Manns, Fabrice; Acosta, Ana Carolina; Parel, Jean-Marie A.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / F. Manns; P.G. Soederberg; A. Ho; B.E. Stuck; M. Belkin. Vol. 5688 2005. p. 19-25 05.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ziebarth, NM, Manns, F, Acosta, AC & Parel, J-MA 2005, Non-contact optical measurement of lens capsule thickness during simulated accommodation. in F Manns, PG Soederberg, A Ho, BE Stuck & M Belkin (eds), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 5688, 05, pp. 19-25, Ophtalmic Technologies XV, San Jose, CA, United States, 1/22/05. https://doi.org/10.1117/12.597648
Ziebarth NM, Manns F, Acosta AC, Parel J-MA. Non-contact optical measurement of lens capsule thickness during simulated accommodation. In Manns F, Soederberg PG, Ho A, Stuck BE, Belkin M, editors, Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 5688. 2005. p. 19-25. 05 https://doi.org/10.1117/12.597648
Ziebarth, Noel Marysa ; Manns, Fabrice ; Acosta, Ana Carolina ; Parel, Jean-Marie A. / Non-contact optical measurement of lens capsule thickness during simulated accommodation. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. editor / F. Manns ; P.G. Soederberg ; A. Ho ; B.E. Stuck ; M. Belkin. Vol. 5688 2005. pp. 19-25
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abstract = "Purpose: To non-invasively measure the thickness of the anterior and posterior lens capsule, and to determine if it significantly changes during accommodation. Methods: Anterior and posterior capsule thickness was measured on post-mortem lenses using a non-contact optical system using a focus-detection technique. The optical system uses a 670nm laser beam delivered to a single-mode fiber coupler. The output of the fiber coupler is focused on the tissue surface using an aspheric lens (NA=0.68) mounted on a translation stage with a motorized actuator. Light reflected from the sample surface is collected by the fiber coupler and sent to a photoreceiver connected to a computer-controlled data acquisition system. Optical intensity peaks are detected when the aspheric lens is focused on the capsule boundaries. The capsule thickness is equal to the distance traveled between two peaks multiplied by the capsule refractive index. Anterior and posterior lens capsule thickness measurements were performed on 18 cynomolgus (age average: 6±1 years, range: 47 years) eyes, 1 rhesus (age: 2 years) eye, and 12 human (age average: 65±16, range: 47-92) eyes during simulated accommodation. The mounted sample was placed under the focusing objective of the optical system so that the light was incident on the center pole. Measurements were taken of the anterior lens capsule in the unstretched and the stretched 5mm states. The lens was flipped, and the same procedure was performed for the posterior lens capsule. Results: The precision of the optical system was determined to be ±0.5μm. The resolution is 4μm and the sensitivity is 52dB. The human anterior lens capsule thickness was 6.0±1.2μm unstretched and 4.9±0.9μm stretched (p=0.008). The human posterior lens capsule was 5.7±1.2μm unstretched and 5.7±1.4μm stretched (p=0.974). The monkey anterior lens capsule thickness was 5.9±1.9μm unstretched and 4.8±1.0μm stretched (p=0.002). The monkey posterior lens capsule was 5.9±2.0μm unstretched and 5.1±1.3μm stretched (p=0.128). Conclusions: The results indicate that the primate anterior lens capsule thickness changes during accommodation.",
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N2 - Purpose: To non-invasively measure the thickness of the anterior and posterior lens capsule, and to determine if it significantly changes during accommodation. Methods: Anterior and posterior capsule thickness was measured on post-mortem lenses using a non-contact optical system using a focus-detection technique. The optical system uses a 670nm laser beam delivered to a single-mode fiber coupler. The output of the fiber coupler is focused on the tissue surface using an aspheric lens (NA=0.68) mounted on a translation stage with a motorized actuator. Light reflected from the sample surface is collected by the fiber coupler and sent to a photoreceiver connected to a computer-controlled data acquisition system. Optical intensity peaks are detected when the aspheric lens is focused on the capsule boundaries. The capsule thickness is equal to the distance traveled between two peaks multiplied by the capsule refractive index. Anterior and posterior lens capsule thickness measurements were performed on 18 cynomolgus (age average: 6±1 years, range: 47 years) eyes, 1 rhesus (age: 2 years) eye, and 12 human (age average: 65±16, range: 47-92) eyes during simulated accommodation. The mounted sample was placed under the focusing objective of the optical system so that the light was incident on the center pole. Measurements were taken of the anterior lens capsule in the unstretched and the stretched 5mm states. The lens was flipped, and the same procedure was performed for the posterior lens capsule. Results: The precision of the optical system was determined to be ±0.5μm. The resolution is 4μm and the sensitivity is 52dB. The human anterior lens capsule thickness was 6.0±1.2μm unstretched and 4.9±0.9μm stretched (p=0.008). The human posterior lens capsule was 5.7±1.2μm unstretched and 5.7±1.4μm stretched (p=0.974). The monkey anterior lens capsule thickness was 5.9±1.9μm unstretched and 4.8±1.0μm stretched (p=0.002). The monkey posterior lens capsule was 5.9±2.0μm unstretched and 5.1±1.3μm stretched (p=0.128). Conclusions: The results indicate that the primate anterior lens capsule thickness changes during accommodation.

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KW - Accommodation

KW - Lens capsule

KW - Presbyopia

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