Noise analysis of ion channels in non-space-clamped cables: Estimates of channel parameters in olfactory cilia

H. P. Larsson, S. J. Kleene, H. Lecar

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Ion channels in the cilia of olfactory neurons are part of the transduction machinery of olfaction. Odorant stimuli have been shown to induce a biphasic current response, consisting of a cAMP-activated current and a Ca2+ -activated CI current. We have developed a noise analysis method to study ion channels in leaky cables, such as the olfactory cilium, under non-space-clamp conditions. We performed steady-state noise analysis on ligand-induced currents in excised cilia, voltage-clamped at input and internally perfused with cAMP or Ca2+. The cAMP-activated channels analyzed by this method gave results similar to those of single-channel recordings (γ = 8.3 pS). Single-channel currents have not yet been recorded for the Ca2+- activated CI channels. Using our noise analysis method, we estimate a unit conductance, γ = 0.8 pS, for these channels. The density of channels was found to be ~70 channels/μm2 for both channel species.

Original languageEnglish (US)
Pages (from-to)1193-1203
Number of pages11
JournalBiophysical journal
Volume72
Issue number3
DOIs
StatePublished - Mar 1997
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics

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