No independent association between HSP70 gene polymorphism and IDDM

Alberto Pugliese, Zuheir L. Awdeh, Aldo Galluzzo, Edmond J. Yunis, Chester A. Alper, George S. Eisenbarth

Research output: Contribution to journalArticle

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Abstract

A role for heat shock proteins (HSPs) in autoimmunity has recently been suggested by several authors. Autoantibodies against HSPs have been associated with such autoimmune diseases as systemic lupus erythematosus, polymyositis, and the NOD mouse model of diabetes. Moreover, genes for the major 70,000-Mr. HSP (HSP70) are located within the MHC. To investigate a potential association of an HSP70-2 gene polymorphism with insulin-dependent diabetes mellitus (IDDM), we analyzed restriction-fragmentlength polymorphism (RFLP) of this gene in 29 families with one or more member affected by IDDM. With the enzyme Pstl, as reported previously, two HSP70-2 alleles of 8.5- and 9.0-kb were found. The 8.5- kb allele was found more frequently on diabetic haplotypes compared with control haplotypes (41 of 66 [62%] vs. 20 of 46 [43%], P = 0.03). This association was due to the conservation of alleles on extended haplotypes we previously reported to be associated with diabetes on initial analysis of families. Twenty-three of 26 diabetic DR3 haplotypes and 3 of 3 normal DR3 haplotypes and all instances of [HLA-B8, SC01, DR3] and [HLA-B18, F1C30, DR3] had the 8.5-kb allele, whereas 0 of 9 normal DR2 haplotypes and 0 of 2 diabetic DR2 haplotypes had the 8.5-kb allele (P = 8 × 10-7 DR3 vs. DR2 haplotypes). The alleles were equally distributed among DR4 haplotypes. Our studies indicate that the 8.5-kb allele is part of [HLA-B8, SC01, DR3] and [HLA-B18, F1C30, DR3] haplotypes, and the 9.0-kb allele is associated with DR2 but no independent association (greater than DR association) is found between HSP70-2 alleles and diabetes.

Original languageEnglish
Pages (from-to)788-791
Number of pages4
JournalDiabetes
Volume41
Issue number7
StatePublished - Jul 1 1992
Externally publishedYes

Fingerprint

Type 1 Diabetes Mellitus
Haplotypes
Alleles
Genes
HLA-B18 Antigen
HLA-B8 Antigen
Heat-Shock Proteins
Polymyositis
Inbred NOD Mouse
HSP70 Heat-Shock Proteins
Autoimmunity
Systemic Lupus Erythematosus
Autoantibodies
Autoimmune Diseases
Enzymes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Pugliese, A., Awdeh, Z. L., Galluzzo, A., Yunis, E. J., Alper, C. A., & Eisenbarth, G. S. (1992). No independent association between HSP70 gene polymorphism and IDDM. Diabetes, 41(7), 788-791.

No independent association between HSP70 gene polymorphism and IDDM. / Pugliese, Alberto; Awdeh, Zuheir L.; Galluzzo, Aldo; Yunis, Edmond J.; Alper, Chester A.; Eisenbarth, George S.

In: Diabetes, Vol. 41, No. 7, 01.07.1992, p. 788-791.

Research output: Contribution to journalArticle

Pugliese, A, Awdeh, ZL, Galluzzo, A, Yunis, EJ, Alper, CA & Eisenbarth, GS 1992, 'No independent association between HSP70 gene polymorphism and IDDM', Diabetes, vol. 41, no. 7, pp. 788-791.
Pugliese A, Awdeh ZL, Galluzzo A, Yunis EJ, Alper CA, Eisenbarth GS. No independent association between HSP70 gene polymorphism and IDDM. Diabetes. 1992 Jul 1;41(7):788-791.
Pugliese, Alberto ; Awdeh, Zuheir L. ; Galluzzo, Aldo ; Yunis, Edmond J. ; Alper, Chester A. ; Eisenbarth, George S. / No independent association between HSP70 gene polymorphism and IDDM. In: Diabetes. 1992 ; Vol. 41, No. 7. pp. 788-791.
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