NMR study of in vivo RIF-1 tumors. Analysis of perchloric acid extracts and identification of 1H, 31P and 13C resonances

William T. Evanochko, Ted T. Sakai, Thian C. Ng, N. Rama Krishna, Hyun Dju Kim, Robert B. Zeidler, Vithal K. Ghanta, R. Wallace Brockman, Lewis M. Schiffer, Paul G. Braunschweiger, Jerry D. Glickson

Research output: Contribution to journalArticle

211 Scopus citations

Abstract

Perchloric acid extracts of radiation-induced fibrosarcoma (RIF-1) tumors grown in mice have been analyzed by multinuclear NMR spectroscopy and by various chromatographic methods. This analysis has permitted the unambiguous assignment of the 31P resonances observed in vivo to specific phosphorus-containing metabolites. The region of the in vivo spectra generally assigned to sugar phosphates has been found in RIF-1 tumors to contain primarily phosphorylethanolamine and phosphorylcholine rather than glycolytic intermediates. Phosphocreatine was observed in extracts of these tumor cells grown in culture as well as in the in vivo spectra, indicating that at least some of the phosphocreatine observed in vivo arises from the tumor itself and not from normal tissues. In the 31P-NMR spectra of the perchloric acid extract, resonances originating from purine and pyrimidine nucleoside di- and triphosphate were resolved. HPLC analyses of the nucleotide pool indicate that adenine derivatives were the most abundant components, but other nucleotides were present in significant amounts. The 1H and 13C resonance assignments of the majority of metabolites present in RIF-1 extracts have also been made. Of particular importance is the ability to observe lactate, the levels of which may provide a noninvasive measure of glycolysis in these cells in both the in vivo and in vitro states. In addition, the aminosulfonic acid, taurine, was found in high levels in the tumor extracts.

Original languageEnglish (US)
Pages (from-to)104-116
Number of pages13
JournalBBA - Molecular Cell Research
Volume805
Issue number1
DOIs
StatePublished - Sep 14 1984

Keywords

  • NMR
  • Perchlorate extract
  • Phosphate analysis
  • Tumor metabolism

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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    Evanochko, W. T., Sakai, T. T., Ng, T. C., Krishna, N. R., Kim, H. D., Zeidler, R. B., Ghanta, V. K., Brockman, R. W., Schiffer, L. M., Braunschweiger, P. G., & Glickson, J. D. (1984). NMR study of in vivo RIF-1 tumors. Analysis of perchloric acid extracts and identification of 1H, 31P and 13C resonances. BBA - Molecular Cell Research, 805(1), 104-116. https://doi.org/10.1016/0167-4889(84)90042-9