NK cells in the CD19- B220+ bone marrow fraction are increased in senescence and reduce E2A and surrogate light chain proteins in B cell precursors

Anne M. King, Patricia Keating, Anjali Prabhu, Bonnie B. Blomberg, Richard L. Riley

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

E2A encoded proteins, key transcriptional regulators in B lineage specification and commitment, have been shown to decrease in B cell precursors in old age. E2A regulates genes encoding the surrogate light chain proteins λ5 and VpreB. In old age, B cell precursors express less surrogate light chain and this results in compromised pre-B cell receptor function and diminished expansion of new pre-B cells in senescence. Herein, we show that aged bone marrow has increased Hardy Fraction A (CD19- B220+) cells, including NK cells, that can inhibit both E47 (E2A) protein and surrogate light chain protein expression in B cell precursors. In vitro, NK-associated inhibition of E47 protein is contact-independent and partially reversed by neutralization of TNFα. In vivo, depletion of NK cells in aged mice by treatment with anti-asialo GM1 antibody led to restoration of surrogate light chain protein levels to that typical of young B cell precursors. These studies suggest that NK cells, within the CD19- B220+ bone marrow cell fraction, may contribute to a bone marrow microenvironment that has the potential to negatively regulate E47 (E2A) as well as surrogate light chain levels in B cell precursors in old age.

Original languageEnglish (US)
Pages (from-to)384-392
Number of pages9
JournalMechanisms of Ageing and Development
Volume130
Issue number6
DOIs
StatePublished - Jun 2009

Keywords

  • B cell precursors
  • Bone marrow microenvironment
  • E2A
  • NK cell

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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