Nitroso-chloramphenicol: Cell cycle specificity of action

A. M. Miller; A. A. Yunis

doi:10.1159/000137577

Nitroso-chloramphenicol: Cell cycle specificity of action

A. M. Miller, A. A. Yunis

Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We have examined the ability of the nitroso derivative of chloramphenicol (NOCAP) to inhibit subsequent growth of logarithmically growing or plateau phase cell cultures after short-term drug exposure. Raji cells taken from log growth cultures exhibited complete inhibition after 6-hour preincubation with 2-3 × 10^-5.M NO-CAP, while similarly treated cells taken from plateau populations demonstrated 90-100% of expected growth on replating. Flow-cytometric studies indicate that the irreversible effects of NO-CAP are greatest as cells proceed into and through the DNA synthetic portion (S phase) of the cell cycle.

Original language	English (US)
Pages (from-to)	61-66
Number of pages	6
Journal	Pharmacology
Volume	24
Issue number	2
DOIs	https://doi.org/10.1159/000137577
State	Published - 1982

Keywords

Cell cycle
Chloramphenicol
Flow cytometry

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1159/000137577

Fingerprint Dive into the research topics of 'Nitroso-chloramphenicol: Cell cycle specificity of action'. Together they form a unique fingerprint.

Cite this

@article{d3758ac4cb3a4054a85d58eb8b605ee5,

title = "Nitroso-chloramphenicol: Cell cycle specificity of action",

abstract = "We have examined the ability of the nitroso derivative of chloramphenicol (NOCAP) to inhibit subsequent growth of logarithmically growing or plateau phase cell cultures after short-term drug exposure. Raji cells taken from log growth cultures exhibited complete inhibition after 6-hour preincubation with 2-3 × 10-5.M NO-CAP, while similarly treated cells taken from plateau populations demonstrated 90-100% of expected growth on replating. Flow-cytometric studies indicate that the irreversible effects of NO-CAP are greatest as cells proceed into and through the DNA synthetic portion (S phase) of the cell cycle.",

keywords = "Cell cycle, Chloramphenicol, Flow cytometry",

author = "Miller, {A. M.} and Yunis, {A. A.}",

year = "1982",

doi = "10.1159/000137577",

language = "English (US)",

volume = "24",

pages = "61--66",

journal = "Pharmacology",

issn = "0031-7012",

publisher = "S. Karger AG",

number = "2",

}

TY - JOUR

T1 - Nitroso-chloramphenicol

T2 - Cell cycle specificity of action

AU - Miller, A. M.

AU - Yunis, A. A.

PY - 1982

Y1 - 1982

N2 - We have examined the ability of the nitroso derivative of chloramphenicol (NOCAP) to inhibit subsequent growth of logarithmically growing or plateau phase cell cultures after short-term drug exposure. Raji cells taken from log growth cultures exhibited complete inhibition after 6-hour preincubation with 2-3 × 10-5.M NO-CAP, while similarly treated cells taken from plateau populations demonstrated 90-100% of expected growth on replating. Flow-cytometric studies indicate that the irreversible effects of NO-CAP are greatest as cells proceed into and through the DNA synthetic portion (S phase) of the cell cycle.

AB - We have examined the ability of the nitroso derivative of chloramphenicol (NOCAP) to inhibit subsequent growth of logarithmically growing or plateau phase cell cultures after short-term drug exposure. Raji cells taken from log growth cultures exhibited complete inhibition after 6-hour preincubation with 2-3 × 10-5.M NO-CAP, while similarly treated cells taken from plateau populations demonstrated 90-100% of expected growth on replating. Flow-cytometric studies indicate that the irreversible effects of NO-CAP are greatest as cells proceed into and through the DNA synthetic portion (S phase) of the cell cycle.

KW - Cell cycle

KW - Chloramphenicol

KW - Flow cytometry

UR - http://www.scopus.com/inward/record.url?scp=0020083019&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020083019&partnerID=8YFLogxK

U2 - 10.1159/000137577

DO - 10.1159/000137577

M3 - Article

C2 - 7079289

AN - SCOPUS:0020083019

VL - 24

SP - 61

EP - 66

JO - Pharmacology

JF - Pharmacology

SN - 0031-7012

IS - 2

ER -

Nitroso-chloramphenicol: Cell cycle specificity of action

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this