Abstract
We have examined the ability of the nitroso derivative of chloramphenicol (NOCAP) to inhibit subsequent growth of logarithmically growing or plateau phase cell cultures after short-term drug exposure. Raji cells taken from log growth cultures exhibited complete inhibition after 6-hour preincubation with 2-3 × 10-5.M NO-CAP, while similarly treated cells taken from plateau populations demonstrated 90-100% of expected growth on replating. Flow-cytometric studies indicate that the irreversible effects of NO-CAP are greatest as cells proceed into and through the DNA synthetic portion (S phase) of the cell cycle.
Original language | English (US) |
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Pages (from-to) | 61-66 |
Number of pages | 6 |
Journal | Pharmacology |
Volume | 24 |
Issue number | 2 |
DOIs | |
State | Published - 1982 |
Keywords
- Cell cycle
- Chloramphenicol
- Flow cytometry
ASJC Scopus subject areas
- Pharmacology