Nitroso-chloramphenicol: Cell cycle specificity of action

A. M. Miller, A. A. Yunis

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We have examined the ability of the nitroso derivative of chloramphenicol (NOCAP) to inhibit subsequent growth of logarithmically growing or plateau phase cell cultures after short-term drug exposure. Raji cells taken from log growth cultures exhibited complete inhibition after 6-hour preincubation with 2-3 × 10-5.M NO-CAP, while similarly treated cells taken from plateau populations demonstrated 90-100% of expected growth on replating. Flow-cytometric studies indicate that the irreversible effects of NO-CAP are greatest as cells proceed into and through the DNA synthetic portion (S phase) of the cell cycle.

Original languageEnglish (US)
Pages (from-to)61-66
Number of pages6
JournalPharmacology
Volume24
Issue number2
DOIs
StatePublished - Jan 1 1982

Keywords

  • Cell cycle
  • Chloramphenicol
  • Flow cytometry

ASJC Scopus subject areas

  • Pharmacology

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